Characterization of the bacterial communities of psyllids associated with Rutaceae in Bhutan by high throughput sequencing.

BMC Microbiology
Jennifer L MorrowPaul Holford

Abstract

Several plant-pathogenic bacteria are transmitted by insect vector species that often also act as hosts. In this interface, these bacteria encounter plant endophytic, insect endosymbiotic and other microbes. Here, we used high throughput sequencing to examine the bacterial communities of five different psyllids associated with citrus and related plants of Rutaceae in Bhutan: Diaphorina citri, Diaphorina communis, Cornopsylla rotundiconis, Cacopsylla heterogena and an unidentified Cacopsylla sp. The microbiomes of the psyllids largely comprised their obligate P-endosymbiont 'Candidatus Carsonella ruddii', and one or two S-endosymbionts that are fixed and specific to each lineage. In addition, all contained Wolbachia strains; the Bhutanese accessions of D. citri were dominated by a Wolbachia strain first found in American isolates of D. citri, while D. communis accessions were dominated by the Wolbachia strain, wDi, first detected in D. citri from China. The S-endosymbionts from the five psyllids grouped with those from other psyllid taxa; all D. citri and D. communis individuals contained sequences matching 'Candidatus Profftella armatura' that has previously only been reported from other Diaphorina species, and the remaining ps...Continue Reading

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Citations

Oct 3, 2020·Microbiology Resource Announcements·Grant A ChambersPaul Holford

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Datasets Mentioned

BETA
AB746416
MN203627
MEGA7

Methods Mentioned

BETA
amplicon sequencing
PCR
electrophoresis

Software Mentioned

Geneious
vegan
R
uclust
Muscle
R environment
QIIME
Statistica
MEGA7
BLAST

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