PMID: 9079Jul 15, 1976

Characterization of the binding of human growth hormone to microsomal membranes from rat liver

The Biochemical Journal
A C HeringtonH G Burger


The binding of 125I-labelled human growth hormone to the 100000g microsomal membrane fraction prepared from the livers of normal female rats was dependent on time, temperature, pH, membrane concentration and concentration of 125I-labelled human growth hormone. At 22 degrees C binding reached a steady state after 16h, with the mean maximal specific binding being 20% of the tracer initially added. Dissociation of 125I-labelled human growth hormone from the membranes, after addition of excess of unlabelled hormone, was relatively slow with a half-time greater than 24h. Only minor degradation of the 125I-labelled human growth hormone was observed during incubation with membranes for 16 or 25h at 22 degrees C. Similarly, no significant change in the ability of membranes to bind human growth hormone was evident after preincubation of the membranes for 16 or 25h. Specificity studies showed that up to 90% of the 125I-labelled human growth hormone bound could be displaced by 1 mug of unlabelled hormone. Ovine prolactin also showed considerable competition for the binding site. Non-primate growth-hormone preparations (ovine, bovine, porcine and rat) and non-related hormones (insulin, thyrotropin, lutropin and follitropin) all showed negl...Continue Reading


Jan 1, 1981·Journal of Receptor Research·A C HeringtonS Ymer
Apr 1, 1979·General and Comparative Endocrinology·M MarquesJ M Dellacha
Oct 1, 1979·General and Comparative Endocrinology·J N Fryer
Feb 1, 1991·General and Comparative Endocrinology·K S GrayK E Nestor
Mar 1, 1981·Metabolism: Clinical and Experimental·R C BaxterJ R Turtle
May 1, 1985·British Journal of Obstetrics and Gynaecology·D L HealyC O'Herlihy
Dec 1, 1994·Acta Paediatrica. Supplement·Q ZhaiF Nyberg
Jul 1, 1986·The Proceedings of the Nutrition Society·L P Milligan, M Summers
Feb 26, 2011·Immunological Investigations·Abdolkhaleg DeezagiNeda Vaseli-Hagh
Nov 10, 2001·American Journal of Physiology. Endocrinology and Metabolism·C G GoodyerG Zogopoulos
Jul 1, 1985·The American Journal of Physiology·J L MessinaJ L Kostyo

Related Concepts

Hydrogen-Ion Concentration
Tissue Membrane
Microsomes, Liver
Recombinant Growth Hormone

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