Characterization of the biological activities of uridine diphosphate in human dendritic cells: Influence on chemotaxis and CXCL8 release

Journal of Cellular Physiology
Marco IdzkoJohannes Norgauer

Abstract

Uridine nucleotides are endogenous nucleotides which are released into the extracellular space from mechanical stressed endothelial and epithelial cells as well as lipopolysaccharide (lps)-stimulated monocytes. Here, we studied the biological activity of the selective purinoreceptor P2Y6 (P2YR6) agonist Uridine 5'diphosphate (UDP) as well as the P2YR2- and P2YR4-activating uridine 5'triphosphate (UTP) on human dendritic cells (DC). These cells in their immature state have the ability to migrate from blood to peripheral target sites where they sense dangerous signals and capture potential antigens. Moreover, mature DC induce innate immune responses and migrate from peripheral tissues to secondary lymphoid organs in order to activate naive T cells and initiate adaptive immunity. Here, we were able to show that uridine nucleotides stimulated Ca(2+) transients, actin polymerization, and chemotaxis in immature DC. Experiments with pertussis toxin, the stable pyrimidine agonist uridine 5'-O-(2-thiodiphosphate) (UDPgammaS) and receptor antagonists, as well as desensitization studies suggested that these uridine nucleotides activities were mediated by different G(i) protein-coupled receptors. During lps-induced maturation, DC lost thei...Continue Reading

References

Aug 16, 1991·Journal of Chromatography·A WernerG Gerber
Sep 1, 1989·Circulation Research·S B Coade, J D Pearson
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Mar 12, 1999·Annual Review of Medicine·J M Timmerman, R Levy
Aug 24, 1999·British Journal of Pharmacology·E R Lazarowski, T K Harden
Feb 7, 2001·The Journal of Biological Chemistry·J K ChambersG P Livi
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M SchnurrA Eigler
Dec 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D FerrariF Di Virgilio
Dec 9, 2000·Naunyn-Schmiedeberg's Archives of Pharmacology·I von Kügelgen, A Wetter
Jan 13, 2001·Current Opinion in Immunology·S Gallucci, P Matzinger
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A la SalaG Girolomoni
Sep 5, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E PantherJ Norgauer
Sep 8, 2001·The Journal of Biological Chemistry·D CommuniJ M Boeynaems
Dec 19, 2001·Circulation·C GoepfertS Robson
Jan 5, 2002·Physiological Reviews·Mary F Lipscomb, Barbara J Masten
Jan 26, 2002·Transplantation·Christophe CauxAlain Vicari
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marco IdzkoJohannes Norgauer
Apr 6, 2002·Circulation Research·Xavier PilloisAlain-Pierre Gadeau
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elisabeth PantherJohannes Norgauer
Nov 5, 2002·Nature Immunology·Miriam MeradEdgar G Engleman
Feb 1, 2003·Trends in Pharmacological Sciences·Maria P AbbracchioGeoffrey Burnstock
Apr 1, 1947·The American Journal of Physiology·H M KALCKAR, O H LOWRY

❮ Previous
Next ❯

Citations

Apr 12, 2008·Purinergic Signalling·Daniel Myrtek, Marco Idzko
Jul 19, 2013·Journal of Molecular Neuroscience : MN·Maosheng Xia, Yue Zhu
Feb 7, 2008·The Journal of Pharmacology and Experimental Therapeutics·Ingrid P FricksT Kendall Harden
Apr 23, 2011·American Journal of Respiratory and Critical Care Medicine·Rodolfo Paula VieiraMarco Idzko
Jul 29, 2006·American Journal of Respiratory Cell and Molecular Biology·Hans BayerMarco Idzko
Apr 1, 2010·Toxins·Susana CubillosKatja Lehmann
May 12, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T MüllerM Idzko
Oct 30, 2014·Purinergic Signalling·Geoffrey Burnstock, Jean-Marie Boeynaems
May 5, 2016·Trends in Immunology·Davide FerrariHolger K Eltzschig
May 16, 2014·Nature·Marco IdzkoHolger K Eltzschig
Aug 12, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Peter StachonAndreas Zirlik
Feb 27, 2007·Journal of Leukocyte Biology·Filip KukulskiJean Sévigny
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel MyrtekMarco Idzko
Jun 3, 2021·Cells·Dominik Klaver, Martin Thurnher
Jun 29, 2021·Frontiers in Immunology·Tom O J CockramGuy C Brown

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.