Characterization of the Borrelia burgdorferi RNase P RNA gene reveals a novel tertiary interaction

Journal of Molecular Biology
J G MattssonL A Kirsebom

Abstract

Characterization of the RNase P RNA gene derived from Borrelia burgdorferi reveals covariation of the conserved nucleotides at positions corresponding to nucleotides 128 and 230 in Escherichia coli RNase P RNA (M1 RNA). Single base substitutions at either of these positions in M1 RNA resulted in a lack of complementation of the temperature-sensitive phenotype associated with rnpA49 in vivo whereas complementation was observed for the double mutant M1 RNA or wild-type M1 RNA. Our in vitro data showed that M1 RNA harbouring a substitution at 128 or 230 cleaved a tRNA precursor both in the absence and presence of C5 with reduced efficiency compared to the wild-type and the double mutant M1 RNA. We conclude that the nucleotides at positions 128 and 230 establish a long-range tertiary interaction in RNase P RNA. Our data also suggest that this interaction together with the identity of the nucleotide at position 230 is important for Pb2+ induced cleavage at specific positions in M1 RNA.

Citations

Jan 1, 1996·Nucleic Acids Research·J Gu, R Reddy
Jul 27, 2005·RNA·Magnus LindellLeif A Kirsebom
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·N E MikkelsenL A Kirsebom
Aug 28, 2007·Journal of Molecular Biology·Mathias BrännvallLeif A Kirsebom
Oct 29, 1998·Journal of Molecular Biology·M BrännvallL A Kirsebom
Jun 1, 1995·Current Opinion in Structural Biology·A M Pyle, J B Green

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