Characterization of the calcium release domains during excitation-contraction coupling in skeletal muscle fibres

Pflügers Archiv : European journal of physiology
Marino DiFrancoJ Vergara

Abstract

The spatiotemporal properties of the Ca2+ release process in skeletal muscle fibres were determined using a confocal spot detection system. The low-affinity, fluorescent Ca2+ indicator Oregon Green 488 BAPTA-5N (OGB-5N) was used to record localized, action potential-induced fluorescence signals from consecutive locations separated by 200 nm within a single sarcomere. Three-dimensional reconstructions of the Ca2+ transients illustrated the existence of fluorescence domains around Ca2+ release sites, which are centred at the T-tubules. By constructing isochronal plots, it was estimated that the earliest detectable full width at half-maximum (FWHM) of the Ca2+ domains was 0.77+/-0.08 microm and increased rapidly with time to 1.4+/-0.04 microm at peak (17-18 degrees C). A delay of 0.64+/-0.1 ms was observed between the onset of the fluorescence transients at the Z- and M-lines. Deconvolution of fluorescence transients gave estimates of approximately 9 and 2 microM for the peak [Ca2+] changes at the Z and M-lines, respectively. Our results are compatible with the possibility that action potential stimulation elicits Ca2+ release from a region of the sarcoplasmic reticulum (SR) broader than the T-SR junction.

Citations

Aug 21, 2007·Pflügers Archiv : European journal of physiology·Pura BolañosCarlo Caputo
Aug 7, 2007·Pflügers Archiv : European journal of physiology·Carlo Caputo, Pura Bolaños
Sep 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Marino DiFrancoJulio L Vergara
Nov 28, 2007·The Journal of General Physiology·Marino DiFrancoJulio L Vergara
Sep 29, 2011·The Journal of General Physiology·Marino DiFranco, Julio L Vergara
Mar 9, 2004·The Journal of Physiology·Christopher E WoodsJulio L Vergara
Jan 26, 2011·The Journal of Physiology·Marino DiFrancoJulio L Vergara
Jul 6, 2010·Progress in Biophysics and Molecular Biology·Stephen M Baylor, Stephen Hollingworth
Aug 16, 2008·The European Journal of Neuroscience·Audrée Pinard, Richard Robitaille
Oct 29, 2003·Biophysical Journal·Fernando D Marengo, Jonathan R Monck
Oct 22, 2008·Methods : a Companion to Methods in Enzymology·R Madelaine ParedesJames D Lechleiter
Feb 26, 2016·Skeletal Muscle·Marino DiFrancoMelissa Jan Spencer

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