PMID: 9529089Apr 7, 1998Paper

Characterization of the carbohydrate moiety of intestinal mucin-type sialoglycoprotein receptors for the K88ac fimbrial adhesin of Escherichia coli

Infection and Immunity
P A GrangeD H Francis

Abstract

We have previously identified two mucin-type sialoglycoproteins from porcine intestinal epithelial cells with approximate molecular masses of 210 (intestinal mucin-type glycoprotein IMTGP-1) and 240 kDa (IMTGP-2) as receptors for the K88ab and K88ac fimbrial adhesins of Escherichia coli. These receptors are detected in intestinal brush border membrane preparations from pigs with adhesive phenotypes but not from pigs with nonadhesive phenotypes and are postulated to be important determinants of the susceptibility of pigs to K88ab+ and K88ac+ enterotoxigenic E. coli infections. Using exoglycosidase digestion studies, we have now determined that beta-linked galactose is an important component in the recognition of IMTGP-1 and IMTGP-2 by the K88ac adhesin. In addition, we observed a differential distribution of the K88ac adhesin binding activity of IMTGP-1 and IMTGP-2 along the crypt-villus axis, suggesting that receptor activity is dependent on the maturation state of the intestinal epithelial cells. Brush borders from immature intestinal epithelial cells possessed the highest concentrations of IMTGP-1 and IMTGP-2 receptor activity, with a progressive decrease in receptor activity as the cells mature. To characterize the differenc...Continue Reading

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Citations

Jul 27, 2007·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·Y WangL Huang
Apr 8, 2015·Journal of Basic Microbiology·Pengpeng XiaGuoqiang Zhu
Aug 11, 2016·Molecular Microbiology·Natalia PakharukovaAnton V Zavialov
Feb 12, 2009·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·J RenL Huang
May 28, 2019·Tropical Animal Health and Production·Rebeka SinhaBharat Bhushan
Sep 17, 2008·Infection and Immunity·Jessica RicciutoPablo Argüeso
Feb 9, 2019·Journal of Animal Physiology and Animal Nutrition·Diana LuisePaolo Trevisi
Jul 15, 1999·Experimental Parasitology·M J KennettL K Riley
Aug 26, 1998·Trends in Microbiology·R A Edwards, J L Puente
Oct 6, 1999·Veterinary Microbiology·M D JeyasinghamD Kelly
Jul 2, 2011·BMC Research Notes·Mette JacobsenClaus B Jørgensen
Dec 23, 2008·International Journal of Biological Macromolecules·Andre-i Sarabia-SainzLuz Vázquez-Moreno

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