Characterization of the cathepsin-like cysteine proteinases of Schistosoma mansoni.

Infection and Immunity
J P DaltonP J Brindley

Abstract

Adult Schistosoma mansoni parasites synthesize and secrete both cathepsin L and cathepsin B cysteine proteinases. These cysteine proteinase activities, believed to be involved in hemoglobin digestion by adult schistosomes, were characterized by using specific fluorogenic peptide substrates and zymography. Both cathepsin L- and B-like activities with pH optima of 5.2 and 6.2, respectively, predominated in soluble extracts of worms, and both these activities were secreted by adult worms into the culture medium. The specific activity of cathepsin L was about double that of cathepsin B when each was assayed at its pH optimum, and moreover, the specific activities of cathepsins L and B in extracts of female schistosomes were 50 to 100% higher than in extracts of male schistosomes. Analysis of the primary structure of two cloned S. mansoni cathepsins L, here termed cathepsin L1 and cathepsin L2, revealed that they are only 44% similar and that cathepsin L2 showed more identity (52%) with human cathepsin L than with schistosome cathepsin L1. Moreover, differences in their active site, propeptide region, and potential for glycosylation suggest separate functions for schistosome cathepsin L1 and cathepsin L2.

References

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Citations

Jan 1, 1997·Annual Review of Microbiology·S E FrancisD E Goldberg
Oct 10, 2006·The Journal of Biological Chemistry·Melaine DelcroixJames H McKerrow
Nov 4, 2009·PLoS Neglected Tropical Diseases·Jennifer M FitzpatrickKarl F Hoffmann
Jan 12, 2001·Oral Microbiology and Immunology·A YamamotoN Goto
Apr 15, 1997·European Journal of Biochemistry·L RocheA McSweeney
Jan 28, 2014·PloS One·Rashika El RidiJohn P Dalton
Dec 12, 2018·Analytical Biochemistry·Elena N ElpidinaIrina Y Filippova
Sep 30, 1998·Gene·C Le BoulayA Van Wormhoudt
Oct 12, 2000·Enzyme and Microbial Technology·A J DowdJ P Dalton
Sep 23, 1997·Molecular and Biochemical Parasitology·P J BrindleyD P McManus
Jun 22, 2002·International Journal for Parasitology·Hiroshi YamasakiTakashi Aoki
Mar 25, 2000·International Journal for Parasitology·G R FrancoE M Rabelo
Jun 5, 2003·International Journal for Parasitology·John P DaltonAlex Loukas
Feb 19, 2008·Molecular and Biochemical Parasitology·Greice Krautz-Peterson, Patrick J Skelly

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