PMID: 9420275Jan 7, 1998Paper

Characterization of the CBF2 binding site within the Epstein-Barr virus latency C promoter and its role in modulating EBNA2-mediated transactivation

Journal of Virology
E M Fuentes-Pananá, P D Ling

Abstract

The Epstein-Barr virus (EBV) EBNA2 protein is a transcriptional activator that regulates viral and cellular gene expression and is also essential for EBV-driven immortalization of B lymphocytes. The EBNA2-responsive enhancer in the viral latency C promoter (Cp) binds two cellular factors, CBF1 and CBF2. The precise role of the CBF2 protein for Cp enhancer function is presently unclear. CBF2 does not appear to interact with EBNA2 and binds just downstream of CBF1 between positions -339 and -368 in the Cp EBNA2 enhancer. Within this region an 8-bp sequence, CAGTGCGT, can be found, and a similar sequence is also located downstream of CBF1 binding sites in other EBNA2-responsive promoters. Previous studies have indicated that mutations and methylation in this sequence affect EBNA2 responsiveness. To investigate the requirements for CBF2 binding, we synthesized a series of oligonucleotides carrying double transversion mutations spanning both the conserved core sequence and outside flanking sequences. Surprisingly, mutations outside of the conserved core sequence in 4 bases immediately flanking the 5' end, GGTT, had the most deleterious effect on CBF2 binding. Mutations in the conserved core had a gradient effect, with those near the...Continue Reading

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J I Cohen
Jan 1, 1991·Journal of Virology·U Zimber-StroblG W Bornkamm
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M WoisetschlaegerS H Speck
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M WoisetschlaegerS H Speck
May 1, 1990·Journal of Virology·S R Kupfer, W C Summers
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R FåhraeusL Rymo
Jul 1, 1989·The Journal of General Virology·M J AlldayB E Griffin
Feb 24, 1995·Cell·E M MiyashitaD A Thorley-Lawson
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·X TongE Kieff
Jun 1, 1995·Journal of Virology·D Marshall, C Sample
Apr 1, 1994·Trends in Microbiology·M G Masucci, I Ernberg
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S R GrossmanE Kieff
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P D LingS D Hayward
Mar 1, 1996·Journal of Virology·T J EvansS Swaminathan
May 1, 1996·Trends in Microbiology·D A Thorley-LawsonG Khan
Jul 1, 1997·Journal of Virology·E M MiyashitaD A Thorley-Lawson

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Citations

Jan 22, 2000·Journal of Virology·S ZhouS D Hayward
Oct 11, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Chisaroka W Echendu, Paul D Ling
May 11, 2000·Molecular and Cellular Biology·S Jeffries, A J Capobianco
May 30, 2003·Molecular and Cellular Biology·Wei-Guo ZhuGregory A Otterson
May 30, 2006·Journal of Virology·Charles M ChauPaul M Lieberman
Dec 24, 2002·The Journal of Biological Chemistry·Janice M AscanoAnthony J Capobianco
Feb 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Matthew P Thompson, Razelle Kurzrock
Nov 5, 1999·Seminars in Cancer Biology·R F AmbinderQ Tao

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