PMID: 7023882Jul 1, 1980Paper

Characterization of the cell cycle and synchrony in a CV-1 cell line

Cytometry
R M D'Alisa, E L Gershey

Abstract

Synchronous populations of CV-1 cells have been prepared by the selective detachment of mitotic cells. An approach, using a combination of standard techniques, has allowed evaluation of the degree of synchrony throughout the cell cycle. CV-1 cells have a cycle of approximately 19-hr duration, throughout which synchronous populations progress with a dispersion of 3 hr, i.e., ie, greater than or equal to 90% of the cells enter S phase and subsequently divide within a 3-hr period. A major cause of this dispersion is the differential attachment times of individual cell within the population when a synchronous culture is initiated.

References

Jan 1, 1979·Cytogenetics and Cell Genetics·R M D'AlisaE L Gershey
Aug 1, 1979·Experimental Cell Research·R M ZuckerE L Gershey
Aug 1, 1979·Experimental Cell Research·E L GersheyR M Zucker
Apr 1, 1979·Journal of Virology·E L Gershey
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J S MurphyF R Landsberger
Jun 1, 1976·Computers and Biomedical Research, an International Journal·J Fried
Dec 12, 1975·Science·B G Durie, S E Salmon
Sep 1, 1973·Experimental Cell Research·J A Robb, K Huebner
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S BrownJ P Revel
Oct 1, 1972·Analytical Biochemistry·R R Klevecz
Apr 1, 1963·Experimental Cell Research·T TERASIMA, L J TOLMACH
Jul 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·F C JENSENH KOPROWSKI

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Citations

Nov 1, 1982·Journal of Virological Methods·J V Scott, P W Choppin
Nov 1, 1990·Experimental Cell Research·D A PanA J Hulbert

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