Characterization of the cell penetrating properties of a human salivary proline-rich peptide

Biochimica Et Biophysica Acta
Giorgia RadicioniAlberto Vitali

Abstract

Saliva contains hundreds of small proline-rich peptides most of which derive from the post-translational and post-secretory processing of the acidic and basic salivary proline-rich proteins. Among these peptides we found that a 20 residue proline-rich peptide (p1932), commonly present in human saliva and patented for its antiviral activity, was internalized within cells of the oral mucosa. The cell-penetrating properties of p1932 have been studied in a primary gingival fibroblast cell line and in a squamous cancer cell line, and compared to its retro-inverso form. We observed by mass-spectrometry, flow cytometry and confocal microscopy that both peptides were internalized in the two cell lines on a time scale of minutes, being the natural form more efficient than the retro-inverso one. The cytosolic localization was dependent on the cell type: both peptide forms were able to localize within nuclei of tumoral cells, but not in the nuclei of gingival fibroblasts. The uptake was shown to be dependent on the culture conditions used: peptide internalization was indeed effective in a complete medium than in a serum-free one allowing the hypothesis that the internalization could be dependent on the cell cycle. Both peptides were inter...Continue Reading

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Citations

Dec 24, 2018·Biotechnology Progress·Adriana AmalfitanoAlessandro Arcovito
Mar 23, 2018·Critical Reviews in Biochemistry and Molecular Biology·Federica IavaroneTiziana Cabras
Dec 13, 2018·International Journal of Analytical Chemistry·Giuseppina NoccaMassimo Cordaro
Sep 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yamil LiscanoJean Paul Delgado
Jul 24, 2018··Alessandro GoriStefano Serra

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