Characterization of the cell wall of a mushroom forming fungus at atomic resolution using solid-state NMR spectroscopy

The Cell Surface
Helena Leona EhrenMarc Baldus

Abstract

Cell walls are essential in the interaction of fungi with the (a)biotic environment and are also key to hyphal morphogenesis and mechanical strength. Here, we used solid-state NMR (ssNMR) spectroscopy combined with HPLC and GC-MS to study the structural organization of the cell wall of a representative of the Basidiomycota, one of the two main phyla of fungi. Based on the data we propose a refined model for the cell wall of a basidiomycete. In this model, the rigid core is built from α- and β-(1,3)-glucan, β-(1,3)-(1,6)-glucan, highly branched and single stranded β-(1,4)-chitin as well as polymeric fucose. The mobile fraction of the cell wall is composed of β-(1,3)-glucan, β-(1,3)-(1,6)-glucan, β-(1,6)-glucan, α-linked reducing and non-reducing ends and polymeric mannose. Together, these findings provide novel insights into the structural organization of the cell wall of the model basidiomycete S. commune that was previously based on destructive chemical and enzymatic analysis.

References

Jan 24, 1977·Biochimica Et Biophysica Acta·J H Sietsma, J G Wessels
Jan 1, 1968·Annual Review of Microbiology·S Bartnicki-Garcia
Jun 1, 1972·Carbohydrate Research·S Bartnicki-Garcia, B Lindberg
Feb 15, 2001·Fungal Genetics and Biology : FG & B·M A van WetterJ G Wessels
Nov 28, 2001·Applied and Environmental Microbiology·H Ait-LahsenA Llobell
Nov 19, 2002·Applied Microbiology and Biotechnology·S P Wasser
May 1, 1962·The Biochemical Journal·E M CROOK, I R JOHNSTON
Sep 15, 2005·Journal of the American Chemical Society·Ovidiu C AndronesiMarc Baldus
Aug 24, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Shaun M Bowman, Stephen J Free
Jun 19, 2007·Mycological Research·David S HibbettNing Zhang
Jul 14, 2010·Nature Biotechnology·Robin A OhmHan A B Wösten
Feb 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marie RenaultMarc Baldus
Aug 1, 2012·Applied Microbiology and Biotechnology·Marijke Haas Jimoh AkanbiKarin Scholtmeijer
Aug 28, 2012·Plant Science : an International Journal of Experimental Plant Biology·Olga SerraRuth E Stark
Dec 4, 2012·The EMBO Journal·Aksana A LabokhaDirk Görlich
Apr 17, 2013·Accounts of Chemical Research·Markus Weingarth, Marc Baldus
Apr 20, 2013·Accounts of Chemical Research·Qing Zhe NiRobert G Griffin
Jan 8, 2015·Applied Microbiology and Biotechnology·Han A B Wösten, Karin Scholtmeijer
Feb 9, 2016·Nature Reviews. Microbiology·Lars P Erwig, Neil A R Gow
Aug 24, 2016·Solid State Nuclear Magnetic Resonance·Tuo WangMei Hong
Jul 18, 2018·Nature Communications·Xue KangTuo Wang
Mar 23, 2019·Current Opinion in Biotechnology·Han A B Wösten

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Methods Mentioned

BETA
chemical treatments
NMR
acetylation
amide resonance
chemical treatment

Software Mentioned

Empower2
INEPT
PeakArea
Waters

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