PMID: 8581967Nov 1, 1995Paper

Characterization of the co-agonist effects of strontium and calcium on myo-inositol trisphosphate-dependent ion fluxes in cerebellar microsomes

Cell Calcium
Z Hannaert-MerahP Champeil

Abstract

Using sheep cerebellum microsomes previously loaded with 45Ca2+ or 90Sr2+, we measured the dependence of inositol 1,4,5-trisphosphate (InsP3)-induced efflux of these ions on Ca2+ or Sr2+ on the cytosolic side. At a low InsP3 concentration, Ca2+ in the submicromolar range only poorly activated 45Ca2+ or 90Sr2+ efflux, and higher Ca2+ concentrations were inhibitory. In contrast, Sr2+ in the micromolar range activated release efficiently, while only very high Sr2+ concentrations were inhibitory. Experiments were repeated in the presence of a high InsP3 concentration, which allowed increasing free Ca2+ to micromolar concentrations without inducing complete inhibition of the InsP3-dependent efflux. Under these conditions, micromolar Ca2+ was found to activate efflux to a large extent, similar to that previously found with Sr2+. Optimal activation by Ca2+ of the InsP3-dependent channel occurs at micromolar rather than submicromolar free Ca2+ concentrations, but at too low an InsP3 concentration, Ca(2+)-induced activation is counteracted by Ca(2+)-induced inactivation. Separate measurements of [3H]-InsP3 binding at a low concentration showed that Sr2+ and Ca2+ did not enhance the amount of bound [3H]-InsP3, implying that the activatin...Continue Reading

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Citations

Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·R E Marquis, A J Hudspeth
Jun 1, 1997·The Journal of General Physiology·D Ogden, T Capiod
Mar 29, 2000·The Journal of General Physiology·S Tertyshnikova, A Fein
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·Geneviève DupontJames W Putney
Apr 13, 2007·Physiological Reviews·J Kevin FoskettDon-On Daniel Mak
Apr 20, 1999·Biological Trace Element Research·E Gonzalez-ReimersF Santolaria-Fernandez
Dec 5, 1998·Biochimica Et Biophysica Acta·C W Taylor
Aug 6, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·O KoukouiL Combettes
Jun 11, 2014·The Journal of Oral Implantology·Thomas B Kardos
Sep 5, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·Min Jeong Ji, Jeong Hee Hong
Nov 5, 1997·The Journal of Biological Chemistry·M J BarreroJ Alvarez

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