Characterization of the complete sequences and stability of plasmids carrying the genes aac(6')-Ib-cr or qnrS in Shigella flexneri in the Hangzhou area of China

World Journal of Microbiology & Biotechnology
Xiao-Ying PuZhe Lu

Abstract

The aim of this study was to explore the fluoroquinolone resistance mechanism of aac (6')-Ib-cr and qnrS gene by comparing complete sequences and stability of the aac(6')-Ib-cr- and qnrS-positive plasmids from Shigella isolates in the Hangzhou area of China. The complete sequences of four newly acquired plasmids carrying aac(6')-Ib-cr or qnrS were compared with those of two plasmids obtained previously and two similar reference Escherichia coli plasmids. The results showed that the length, antibiotic resistance genes and genetic environment were different among the plasmids. Moreover, the plasmid stability of three wild-type isolates and five plasmid transformants carrying aac(6')-Ib-cr and/or qnrS was measured in vitro, and all eight isolates were found to have lost their aac(6')-Ib-cr- or qnrS-positive plasmids to a different extent at different stages. When the plasmids were electroporated into Shigella flexneri or they lost positive plasmids, the MICs of ciprofloxacin increased or decreased two- to eightfold for aac(6')-Ib-cr-positive plasmids and 16- to 32-fold for qnrS-positive plasmids. To our knowledge, this is the first report comparing the complete sequences and describing stability for the aac(6')-Ib-cr- and qnrS-pos...Continue Reading

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Citations

Oct 12, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Wen-Xia ZhangJue Zhang
Jun 23, 2021·International Journal of Antimicrobial Agents·Albert Moreno-MingoranceJuan José González-López

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Datasets Mentioned

BETA
GU371928
KF362122
KJ201887
CP012139
CP012141
CP012734
CP012143

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST

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