PMID: 8597573Feb 8, 1996Paper

Characterization of the constituent polypeptides of the extracellular hemoglobin from Lumbricus terrestris: heterogeneity and discovery of a new linker chain L4

Biochimica Et Biophysica Acta
Kenzo FushitaniK Hosokawa

Abstract

The extracellular hemoglobin of Lumbricus terrestris comprises four oxygen binding chains, a, b, c, d, and three linker chains L1, L2, L3 as major components. A stoichiometry of the whole molecule has been proposed on the basis of these chains, with a total number of 216 chains: forty-eight chains of each oxygen binding chain and eight molecules of each linker chain. We have isolated additional minor components by HPLC and two-dimensional gel electrophoresis. The following biochemical characterizations have been made. (i) All components so far reported, the heme-containing chains a, b, c, d, and linker chains L1, L2, L3 and a new minor polypeptide, L4, were mapped on a two-dimensional gel. Their estimated isoelectric points were between 4.7 and 5.9. (ii) The sequences of several peptides including the unique N-terminal peptide from linker L4 show that it can be considered as a duplicated gene product with a similar mass. (iii) Chain d2 was isolated and found to correspond to the minor chain previously pointed out by Shishikura et al. (J. Biol. Chem. 262 (1987) 3123-3131). (iv) The major chain d1 has serine at position 7 from the N-terminus. This is not consistent with previously reported glycine (Shishikura et al., J. Biol. Che...Continue Reading

References

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Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D Stoppa-LyonnetM Tosi
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Dec 29, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Danqing XiaoMichael A Schwarzschild

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Citations

Mar 24, 2004·Micron : the International Research and Review Journal for Microscopy·Serge N Vinogradov
Nov 13, 2007·International Journal of Biological Macromolecules·Marilene Silva OliveiraMarcel Tabak
Nov 22, 1996·The Journal of Biological Chemistry·H ZhuA F Riggs
Mar 29, 2001·Physiological Reviews·R E Weber, S N Vinogradov

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