Characterization of the core oligosaccharide and the O-antigen biological repeating unit from Halomonas stevensii lipopolysaccharide: the first case of O-antigen linked to the inner core

Chemistry : a European Journal
Giuseppina PierettiM Michela Corsaro

Abstract

A novel core structure among bacterial lipopolysaccharides (LPS) that belong to the genus Halomonas has been characterized. H. stevensii is a moderately halophilic microorganism, as are the majority of the Halomonadaceae. It brought to light the pathogenic potential of this genus. On account of their role in immune system elicitation, elucidation of LPS structure is the mandatory starting point for a deeper understanding of the interaction mechanisms between host and pathogen. In this paper we report the structure of the complete saccharidic portion of the LPS from H. stevensii. In contrast to the finding that the O-antigen is usually covalently linked to the outer core oligosaccharide, we could demonstrate that the O-polysaccharide of H. stevensii is linked to the inner core of an LPS. By means of high-performance anion-exchange chromatography with pulsed amperometric detection we were able to isolate the core decasaccharide as well as a tridecasaccharide constituted by the core region plus one O-repeating unit after alkaline degradation of the LPS. The structure was elucidated by one- and two-dimensional NMR spectroscopy, ESI Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry, and chemical analysis.

References

Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Jun 1, 1969·European Journal of Biochemistry·C GalanosO Westphal
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Aug 26, 1996·Carbohydrate Research·A D CoxM B Perry
Aug 2, 2000·Journal of Clinical Microbiology·A von GraevenitzM Ritzler
Oct 3, 2001·Journal of Endotoxin Research·C Alexander, E T Rietschel
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Dec 13, 2003·Carbohydrate Research·Martine Caroff, Doris Karibian
Mar 24, 2005·Infection and Immunity·Marta Biedzka-SarekMikael Skurnik
Dec 3, 2005·European Journal of Mass Spectrometry·Anna Kondakov, Buko Lindner
Aug 28, 2007·The Journal of Hospital Infection·P BergerM Drancourt
Aug 5, 2009·International Journal of Systematic and Evolutionary Microbiology·Kwang Kyu KimJung-Sook Lee
Dec 21, 2010·Carbohydrate Research·Giuseppina PierettiM Michela Corsaro

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