PMID: 2110449Apr 15, 1990Paper

Characterization of the cysteine residues and disulphide linkages in the protein crystal of Bacillus thuringiensis

The Biochemical Journal
H P BietlotH Kaplan

Abstract

Bacillus thuringiensis produces a 130-140 kDa insecticidal protein in the form of a bipyramidal crystal. The protein in the crystals from the subspecies kurstaki HD-1 and entomocidus was found to contain 16-18 cysteine residues per molecule, present primarily in the disulphide form as cystine. Evidence that all the cysteine residues form symmetrical interchain disulphide linkages in the protein crystal was obtained from the following results: (i) the disulphide diagonal procedure [Brown & Hartley (1966) Biochem. J. 101, 214-228] gave only unpaired cysteic acid peptides in diagonal maps; (ii) the disulphide bridges were shown to be labile in dilute alkali and the crystal protein could be released quantitatively with 1 mM-2-mercaptoethanol; (iii) the thiol groups of the released crystal protein were shown by competitive labelling [Kaplan, Stevenson & Hartley (1971) Biochem. J. 124, 289-299] to have the same chemical properties as exposed groups on the surface of the protein; (iv) the thiol groups in the released crystal protein reacted quantitatively with iodoacetate or iodoacetamide. The finding that all the disulphide linkages in the protein crystal are interchain and symmetrical accounts for its alkali-lability and for the hig...Continue Reading

Citations

Mar 15, 1994·FEMS Microbiology Letters·A I Aronson
Aug 20, 2016·Applied Microbiology and Biotechnology·Raida Zribi ZghalSlim Tounsi
Jun 13, 2018·Biological Chemistry·Robert SchönherrLars Redecke
Mar 20, 2010·Journal of the American Society for Mass Spectrometry·Jennifer Nguyen, Scott C Russell
May 16, 1998·The Journal of Biological Chemistry·F R ClairmontH Kaplan
Oct 27, 2001·Applied and Environmental Microbiology·L ChangA Aronson
Jul 3, 2020·Toxins·Mikel Domínguez-ArrizabalagaPrimitivo Caballero
Mar 12, 2017·Toxins·Rooma AdalatAbdul Rauf Shakoori
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·E SchnepfD H Dean
Jul 3, 2021·Toxins·Guillaume TetreauJacques-Philippe Colletier

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