Characterization of the cytokine and maturation responses of pure populations of porcine plasmacytoid dendritic cells to porcine viruses and toll-like receptor agonists.

Veterinary Immunology and Immunopathology
Gabriela Calzada-NovaFederico A Zuckermann

Abstract

Plausible representatives of plasmacytoid dendritic cells (pDCs) in pigs have been characterized as being CD4(hi)CD172(lo). Due to their paucity in blood, we utilized novel fluorescent-activated cell sorting procedures to isolate them from PBMC. The resultant subset was greater than 98% homogeneous in regards to the selected phenotype and contained the preponderance of individuals secreting IFN-alpha after exposure to a known stimulant, transmissible gastroenteritis virus (TGEV). In addition to being a potent source of IFN-alpha, other properties of these porcine CD4(hi)CD172(lo) cells including their morphological transition from a plasma cell-like shape during quiescence to one resembling a dendritic cell (DC) after activation by TGEV and their relatively strong constitutive expression of interferon regulatory factor-7 (IRF-7) conformed to the expectations of genuine pDCs. While a substantial IFN-alpha response was also elicited from the porcine pDCs by pseudorabies virus (PrV), swine influenza virus (SIV), and TLR7 and 9 agonists, there was an agent-dependent induction of varying amounts of IL-2, IL-6, IL-8, IL-12, IFN-gamma, and TNF-alpha. Notably, porcine reproductive and respiratory syndrome virus (PRRSV) failed to provok...Continue Reading

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Citations

Jul 5, 2013·Journal of Virology·Jazmina L G CruzSonia Zúñiga
Dec 14, 2011·Animal Health Research Reviews·Yongming SangFrank Blecha
Sep 21, 2010·Virus Research·Roongroje Thanawongnuwech, Sanipa Suradhat
Aug 12, 2014·Virology·Martina BecaresSonia Zuñiga
Jul 26, 2015·Veterinary Immunology and Immunopathology·Crystal L LovingFederico A Zuckermann
Apr 9, 2014·Developmental and Comparative Immunology·K H MairF Meurens
Nov 12, 2014·Annual Review of Animal Biosciences·Artur SummerfieldMeret Ricklin
Apr 12, 2013·PloS One·Nils Lannes, Artur Summerfield
Mar 14, 2019·Veterinary Sciences·Elisa CrisciMaria Montoya
Mar 8, 2019·Frontiers in Veterinary Science·Sergio Montaner-TarbesLorenzo Fraile
Mar 28, 2017·Veterinary Microbiology·Hanzhong Ke, Dongwan Yoo
Aug 17, 2021·Frontiers in Immunology·Jesús HernándezEnric Mateu

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Methods Mentioned

BETA
ubiquitination
fluorescent-activated cell sorting
ELISA
light microscopy
FACS
flow cytometry

Software Mentioned

FlowJo

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