PMID: 9525573Apr 3, 1998Paper

Characterization of the DNA-binding domain of the bovine papillomavirus replication initiator E1

Journal of Virology
G Chen, A Stenlund

Abstract

The bovine papillomavirus replication initiator protein E1 is an origin of replication (ori)-binding protein absolutely required for viral DNA replication. In the presence of the viral transcription factor E2, E1 binds to the ori and initiates DNA replication. To understand how the E1 initiator recognizes the ori and how E2 assists in this process, we have expressed and purified a 166-amino-acid fragment which corresponds to the minimal E1 DNA-binding domain (DBD). DNA binding studies using this protein demonstrate that the E1 DBD can bind to the palindromic E1 binding site in several forms but that binding of two monomers, each recognizing one half-site of the E1 palindrome, is the predominant form. This is reminiscent of the binding of the T-antigen DBD to the SV40 ori, and interestingly, the arrangement of E1 binding sites shows striking similarities to the arrangement of T-antigen binding sites in the SV40 ori even though the recognition sequences are unrelated. The E1 DBD is capable of interacting cooperatively with E2; however, the E2 DBD and not the E2 activation domain mediates this interaction. Furthermore, the E2 DBD stimulates binding of two monomers of the E1 DBD to the ori by binding cooperatively with one E1 monom...Continue Reading

References

Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L Z ChengT J Kelly
Jun 1, 1992·Molecular and Cellular Biology·Z S Guo, M L DePamphilis
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M ChiangL T Chow
Jan 1, 1992·Annual Review of Biochemistry·E Fanning, R Knippers
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M Lusky, E Fontane
Nov 22, 1991·Science·H zur Hausen
Feb 1, 1992·Molecular and Cellular Biology·R Aurora, W Herr
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Dec 1, 1995·Journal of Virology·T MelendyA Stenlund
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·R Li, M R Botchan
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·T G GilletteJ A Borowiec
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·M LuskyY S Seo
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y S SeoJ Hurwitz
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·E UstavA Stenlund
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y S SeoJ Hurwitz
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L YangM Botchan
May 1, 1997·Journal of Virology·M Berg, A Stenlund

❮ Previous
Next ❯

Citations

Feb 6, 1999·The Journal of Biological Chemistry·E T FoutsM R Botchan
Jul 19, 2000·The Journal of General Virology·G D McShan, V G Wilson
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Karl E Duderstadt, James M Berger
May 3, 2013·Journal of Virology·Stephen SchuckArne Stenlund
Feb 6, 2016·BMC Evolutionary Biology·Beatriz Mengual-ChuliáIgnacio G Bravo
Nov 1, 2003·The Journal of Biological Chemistry·Anitra S Auster, Leemor Joshua-Tor
Jan 18, 2006·Journal of Virology·Randolph V ClowerThomas Melendy
Mar 26, 2005·The Biochemical Journal·Anna Zawilak-PawlikJolanta Zakrzewska-Czerwińska
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Seung-Jae LeeLeemor Joshua-Tor
Dec 23, 2016·Scientific Reports·Io Nam WongCyril M Sanders
Sep 25, 2003·Nature Reviews. Molecular Cell Biology·Arne Stenlund
Jun 16, 2005·Journal of Virology·Stephen Schuck, Arne Stenlund
Jul 16, 2013·Virology·Alison A McBride

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.