Characterization of the Drosophila adenosine receptor: the effect of adenosine analogs on cAMP signaling in Drosophila cells and their utility for in vivo experiments

Journal of Neurochemistry
Lucie KucerovaMichal Zurovec

Abstract

Adenosine receptors (AR) belonging to the G protein-coupled receptor family influence a wide range of physiological processes. Recent elucidation of the structure of human A2AR revealed the conserved amino acids necessary for contact with the Ado moiety. However, the selectivity of Ado analogs for AR subtypes is still not well understood. We have shown previously that the Drosophila adenosine receptor (DmAdoR) evokes an increase in cAMP and calcium concentration in heterologous cells. In this study, we have characterized the second-messenger stimulation by endogenous DmAdoR in a Drosophila neuroblast cell line and examined a number of Ado analogs for their ability to interact with DmAdoR. We show that Ado can stimulate cAMP but not calcium levels in Drosophila cells. We found one full and four partial DmAdoR agonists, as well as four antagonists. The employment of the full agonist, 2-chloroadenosine, in flies mimicked in vivo the phenotype of DmAdoR over-expression, whereas the antagonist, SCH58261, rescued the flies from the lethality caused by DmAdoR over-expression. Differences in pharmacological effect of the tested analogs between DmAdoR and human A2AR can be partially explained by the dissimilarity of specific key amino a...Continue Reading

References

Nov 16, 1992·Biochemical and Biophysical Research Communications·C SamakovlisD Hultmark
Jan 1, 1991·Progress in Neurobiology·R W Greene, H L Haas
Aug 1, 1994·Trends in Pharmacological Sciences·M R BoarderG A Weisman
Apr 1, 1994·In Vitro Cellular & Developmental Biology. Animal·K UiT Miyake
Jul 26, 2000·The Journal of Cell Biology·T Brody, A Cravchik
Aug 24, 2001·Archives of Insect Biochemistry and Physiology·J V Broeck
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michal ZurovecPeter J Bryant
Oct 31, 2003·Journal of Medicinal Chemistry·Soo-Kyung KimKenneth A Jacobson
Dec 10, 2003·Expert Opinion on Emerging Drugs·Luo YanChrista E Müller
Jul 20, 2004·Nature Neuroscience·Doo-Sup ChoiRobert O Messing
Sep 25, 2004·Journal of Cellular Physiology·R YaarKatya Ravid
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·Bertil B FredholmJean-Marie Vaugeois
May 24, 2005·PLoS Biology·Tomas DolezalPeter J Bryant
Nov 5, 2005·Cell·Dragana Rogulja, Kenneth D Irvine
Oct 27, 2006·The Journal of Pharmacology and Experimental Therapeutics·P MichalV Dolezal
Mar 21, 2007·Insect Biochemistry and Molecular Biology·Eva DolezelovaMichal Zurovec
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Sep 7, 2007·Progress in Neurobiology·Bertil B FredholmMichail Sitkovsky
Jun 24, 2008·ACS Chemical Biology·Frank FanKeith V Wood
Sep 2, 2008·Nature Reviews. Drug Discovery·György HaskóPál Pacher
Sep 19, 2009·Trends in Pharmacological Sciences·Riccardo Polosa, Michael R Blackburn
Feb 16, 2010·Experimental Cell Research·Bertil B Fredholm
Apr 8, 2010·Current Topics in Medicinal Chemistry·M L TrincavelliC Martini
Apr 8, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David KnightGabrielle L Boulianne
Jul 3, 2010·Biochimica Et Biophysica Acta·Henni PiirainenVeli-Pekka Jaakola
Dec 28, 2010·Biochimica Et Biophysica Acta·Christa E Müller, Kenneth A Jacobson
Mar 12, 2011·Science·Fei XuRaymond C Stevens
Jun 1, 1990·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·David J Peel, Martin J Milner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

© 2022 Meta ULC. All rights reserved