Characterization of the Drosophila caspase, DAMM

The Journal of Biological Chemistry
N L HarveyS Kumar

Abstract

Caspases are main effectors of apoptosis in metazoans. Genome analysis indicates that there are seven caspases in Drosophila, six of which have been previously characterized. Here we describe the cloning and characterization of the last Drosophila caspase, DAMM. Similar to mammalian effector caspases, DAMM lacks a long prodomain. We show that the DAMM precursor, along with the caspases DRONC and DECAY, is partially processed in cells undergoing apoptosis. Recombinant DAMM produced in Escherichia coli shows significant catalytic activity on a pentapeptide caspase substrate. Low levels of damm mRNA are ubiquitously expressed in Drosophila embryos during early stages of development. Relatively high levels of damm mRNA are detected in larval salivary glands and midgut, and in adult egg chambers. Ectopic expression of DAMM in cultured cells induces apoptosis, and similarly, transgenic overexpression of DAMM, but not of a catalytically inactive DAMM mutant, in Drosophila results in a rough eye phenotype. We demonstrate that expression of the catalytically inactive DAMM mutant protein significantly suppresses the rough eye phenotype due to the overexpression of HID, suggesting that DAMM may be required in a hid-mediated cell death pat...Continue Reading

References

Apr 11, 1997·The Journal of Biological Chemistry·R V TalanianW W Wong
May 16, 1997·The Journal of Biological Chemistry·N L HarveyS Kumar
May 15, 1997·The EMBO Journal·A G Fraser, G I Evan
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·N InoharaG Núñez
Jan 31, 1998·Science·K McCall, H Steller
Jun 5, 1998·The Journal of Biological Chemistry·K F HarveyS Kumar
Jun 17, 1998·Genes & Development·V Cryns, J Yuan
Aug 28, 1998·Science·D R Green, J C Reed
Oct 3, 1998·The Journal of Biological Chemistry·P A ColussiS Kumar
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·H KanukaM Miura
Feb 17, 1999·Genes & Development·Q L Deveraux, J C Reed
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·C J HawkinsB A Hay
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L DorstynS Kumar
Oct 16, 1999·The Journal of Biological Chemistry·L DorstynS Kumar
Dec 1, 1999·Cell Death and Differentiation·D W Nicholson
Dec 1, 1999·Cell Death and Differentiation·T S ZhengR A Flavell
Dec 1, 1999·Cell Death and Differentiation·H R Stennicke, G S Salvesen
Dec 1, 1999·Cell Death and Differentiation·S Kumar
Dec 1, 1999·Cell Death and Differentiation·P G EkertD L Vaux
Feb 17, 2000·The EMBO Journal·P MeierG I Evan
Jul 19, 2000·Current Biology : CB·M Elrod-EricksonD Schneider
Sep 14, 2000·The Journal of Biological Chemistry·L M QuinnH Richardson
Jan 4, 2001·Cell Death and Differentiation·S Kumar, J Doumanis
Jan 4, 2001·Cell Death and Differentiation·B A Hay
Mar 21, 2001·Cell·J ChaiP Dataa
Mar 21, 2001·Cell·S J RiedlG S Salvesen
Jun 27, 2009·Annual Review of Neuroscience·Arnold Kriegstein, Arturo Alvarez-Buylla

❮ Previous
Next ❯

Citations

Feb 13, 2009·Apoptosis : an International Journal on Programmed Cell Death·Dawn M CooperCarl Lowenberger
Jul 30, 2003·Developmental Biology·J S PetersonK McCall
Jun 20, 2002·Journal of Immunological Methods·Helena Richardson, Sharad Kumar
Nov 4, 2006·Cell Death and Differentiation·S Kumar
Apr 13, 2004·Genes & Development·Angelike StathopoulosMichael Levine
Oct 14, 2004·Science's STKE : Signal Transduction Knowledge Environment·Sharad Kumar
Sep 19, 2006·Molecular and Cellular Biology·Shu KondoMasayuki Miura
Jul 18, 2006·Annual Review of Cell and Developmental Biology·Bruce A Hay, Ming Guo
Jan 30, 2014·PloS One·Katherine TaylorDeborah A Kimbrell
Feb 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Marco Di FruscioStephane Richard
Mar 31, 2005·Seminars in Cell & Developmental Biology·Peter CashioAndreas Bergmann
Feb 22, 2012·The Journal of Cell Biology·Anat Florentin, Eli Arama
Mar 20, 2002·The Journal of Cell Biology·Loretta DorstynSharad Kumar
May 6, 2009·Genesis : the Journal of Genetics and Development·Claudio R SimonRicardo Guelerman P Ramos
Dec 8, 2004·International Journal for Parasitology·Hilary Hurd, Victoria Carter
Jun 20, 2015·Genetics Research International·Kimberly A CarlsonLawrence G Harshman
Mar 19, 2015·Journal of Insect Physiology·A AccorsiD Malagoli
Oct 13, 2009·Current Biology : CB·Donna DentonSharad Kumar
Mar 5, 2004·Biochimica Et Biophysica Acta·Tatsushi Igaki, Masayuki Miura
Jul 3, 2013·Biochimica Et Biophysica Acta·Donna DentonSharad Kumar
Oct 7, 2004·Developmental Biology·Kristen Cullen, Kimberly McCall
Apr 25, 2013·Biochemical and Biophysical Research Communications·Dong Hoon LeeSo Hee Kwon
Feb 24, 2016·The European Journal of Neuroscience·Juliane Melzer, Meike Broemer
Jun 24, 2004·The Journal of Biological Chemistry·Ayako Kohyama-KoganeyaYoshio Hirabayashi
Sep 10, 2003·International Reviews of Immunology·Thomas E Clarke, Rollie J Clem
Dec 3, 2003·Cell Death and Differentiation·M BoyceJ Yuan
Jun 5, 2003·The Journal of Biological Chemistry·Raju IlangovanJianhua Zhou
Dec 3, 2003·The Journal of Biological Chemistry·Charles M ForsythAndrew J Fisher
Apr 13, 2004·Oncogene·Richard A Lockshin, Zahra Zakeri
Nov 21, 2019·Cell Death and Differentiation·Tianqi XuSharad Kumar
Nov 17, 2017·G3 : Genes - Genomes - Genetics·Wayne HemphillMatthew Talbert
Oct 10, 2003·Development·Jaime Mergliano, Jonathan S Minden
Sep 24, 2004·Journal of Cell Science·Israel MuroRollie J Clem
Dec 12, 2003·Development·Damali N Martin, Eric H Baehrecke
Apr 30, 2005·Journal of Cell Science·Sally Kornbluth, Kristin White

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