PMID: 7543477Aug 1, 1995Paper

Characterization of the duplicate ribulose-1,5-bisphosphate carboxylase genes and cbb promoters of Alcaligenes eutrophus

Journal of Bacteriology
B KusianB Bowien

Abstract

Autotrophic CO2 fixation via the Calvin carbon reduction cycle in Alcaligenes eutrophus H16 is genetically determined by two highly homologous cbb operons, one of which is located on the chromosome and the other on megaplasmid pHG1 of the organism. An activator gene, cbbR, lies in divergent orientation only 167 bp upstream of the chromosomal operon and controls the expression of both cbb operons. The two 5'-terminal genes of the operons, cbbLS, coding for ribulose-1,5-bisphosphate carboxylase/oxygenase, were sequenced. Mapping of the 5' termini of the 2.1-kb cbbLS transcripts by primer extension and by nuclease S1 treatment revealed a single transcriptional start point at the same relative position for the chromosomal and plasmid-borne cbb operons. The derived cbb operon promoter showed similarity to sigma 70-dependent promoters of Escherichia coli. For the 1.4-kb transcripts of cbbR, the transcriptional start points were different in autotrophic and heterotrophic cells. The two corresponding cbbR promoters overlapped the cbb operon promoter and also displayed similarities to sigma 70-dependent promoters. The deficient cbbR gene located on pHG1 was transcribed as well. A newly constructed double operon fusion vector was used to...Continue Reading

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Citations

Jan 19, 1999·Annual Review of Microbiology·J M ShivelyW G Meijer
Mar 31, 2000·Molecular Biology and Evolution·U G MaierS Douglas
Dec 31, 1997·FEMS Microbiology Reviews·B Kusian, B Bowien
Jun 9, 2004·Microbiology·Xueming WeiDaniel J Arp
Sep 2, 2015·Journal of Bacteriology·Andrew W Dangel, F Robert Tabita
Sep 4, 2012·Applied and Environmental Microbiology·Steffen LütteBärbel Friedrich
Feb 7, 2003·Applied and Environmental Microbiology·Elke B Heinz, Wolfgang R Streit
Aug 24, 2002·Journal of Bacteriology·Bernhard KusianBotho Bowien
Feb 8, 2006·Applied and Environmental Microbiology·Olivia GohlBeate Averhoff
Apr 1, 1997·Microbiology·Claudia GrzeszikHans G Schlegel

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