Characterization of the dystrophin-syntrophin interaction using the two-hybrid system in yeast

FEBS Letters
A CastellóH Gilgenkrantz

Abstract

The carboxy-terminal region of dystrophin has previously been shown to interact directly with alpha1 syntrophin, a cytoplasmic component of the dystrophin-glycoprotein complex, by in vitro biochemical studies such as overlay assay or immunoprecipitation. Using the two-hybrid system, we have isolated from a human heart cDNA library the entire coding sequence of human alpha1 syntrophin, therefore confirming for the first time this interaction via an in vivo approach. In addition, we have reduced the interaction domain to the distal half of alpha1 syntrophin.

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Citations

Sep 23, 2003·Journal of Molecular Biology·Karine GrisoniLaurent Ségalat
Jan 23, 1998·Nature Structural Biology·J SchultzH Oschkinat
Jan 19, 2011·The Journal of Cell Biology·Shan Zhou, Lihsia Chen
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Apr 5, 2000·The Journal of Biological Chemistry·G PilusoV Nigro
Dec 6, 2018·Cell Proliferation·Sahar S BhatFirdous A Khanday

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