Characterization of the effects of trace concentrations of graphene oxide on zebrafish larvae through proteomic and standard methods

Ecotoxicology and Environmental Safety
Wei ZouXiangang Hu

Abstract

The effects of graphene oxide (GO) carbon nanomaterials on ecosystems have been well characterized, but the toxicity of GO at predicted environmental concentrations to living organisms at the protein level remain largely unknown. In the present work, the adverse effects and mechanisms of GO at predicted environmental concentrations were evaluated by integrating proteomics and standard analyses for the first time. The abundances of 243 proteins, including proteins involved in endocytosis (e.g., cltcb, arf6, capzb and dnm1a), oxidative stress (e.g., gpx4b, sod2, and prdx1), cytoskeleton assembly (e.g., krt8, krt94, lmna and vim), mitochondrial function (e.g., ndufa10, ndufa8, cox5aa, and cox6b1), Ca2+ handling (e.g., atp1b2a, atp1b1a, atp6v0a1b and ncx4a) and cardiac function (e.g., tpm4a, tpm2, tnni2a.1 and tnnt3b), were found to be notably altered in response to exposure 100 μg/L GO. The results revealed that GO caused malformation and mortality, likely through the downregulation of proteins related to actin filaments and formation of the cytoskeleton, and induced oxidative stress and mitochondrial disorders by altering the levels of antioxidant enzymes and proteins associated with the mitochondrial membrane respiratory chain. ...Continue Reading

Citations

Jul 7, 2019·Fish Physiology and Biochemistry·Jaqueline P SouzaValtencir Zucolotto
Dec 15, 2018·Environmental Toxicology·Xiaole YangZhengwei Fu
Jan 1, 2019·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Anisha AnandChih-Ching Huang
Jan 25, 2020·Dose-response : a Publication of International Hormesis Society·Chuanrui ZhengThomas Efferth
Sep 3, 2018·The Science of the Total Environment·Ying ZhangXie Quan
Nov 13, 2019·The Science of the Total Environment·Daming WuTing Zhang
Feb 23, 2021·The Science of the Total Environment·Ignacio Martínez-ÁlvarezAmaia Orbea
Aug 23, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Binbin LiRunguang Sun
Apr 20, 2020·Ecotoxicology and Environmental Safety·Zhong ChenMing Yang

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