Characterization of the epidermal growth factor receptor in the gastric mucosa

Digestion
B L SlomianyA Slomiany

Abstract

The effects of epidermal growth factor (EGF), a potent mitogen involved in mucosal protection, are mediated by specific cellular receptors. Here, we present the characteristics and binding properties of EGF receptors in the gastric mucosa. The studies were conducted using cell membranes isolated by subcellular fractionation of rat stomach mucosal scrapings. Specific binding of [125I]-EGF to the membrane preparations was assessed at room temperature for various periods of time and at different pHs. The results showed that the binding was proportional to the incubation time up to 1 h and was not affected by a pH change between 4.0 and 7.0. Scatchard analysis of the binding data infer the presence of 2 binding sites, one of high affinity (Kd = 1.34 nM, Bmax = 34 fmol/mg protein) and the other of low affinity (Kd = 484 nM, Bmax = 2.29 pmol/mg protein). Cross-linking experiments using disuccinimidyl suberate to link the [125I]-EGF to gastric membranes followed by polyacrylamide gel electrophoresis and autoradiography revealed that the major receptor for EGF was a protein of 170 kilodaltons. When the solubilized membranes were subjected to wheat germ agglutinin affinity chromatography, the purified material was found to act as substr...Continue Reading

Citations

Aug 1, 1992·The International Journal of Biochemistry·B L SlomianyA Slomiany
Jun 1, 1993·The International Journal of Biochemistry·B L SlomianyA Slomiany
Jan 1, 1993·The International Journal of Biochemistry·J LiuB L Slomiany
Sep 1, 1992·General Pharmacology·B L SlomianyA Slomiany
Nov 1, 1992·General Pharmacology·J LiuB L Slomiany
May 1, 1994·General Pharmacology·B L SlomianyA Slomiany
Dec 1, 1992·The Journal of Clinical Investigation·M RomanoR J Coffey
Aug 14, 1992·Biochemical and Biophysical Research Communications·B L SlomianyA Slomiany
May 15, 2013·International Journal of Molecular Sciences·Luciana H Osaki, Patrícia Gama
Aug 1, 1992·Scandinavian Journal of Gastroenterology·S J KonturekB L Slomiany
Dec 1, 2010·Molecular and Cellular Biochemistry·S I ChandranathJ Singh

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