PMID: 6351916Aug 16, 1983Paper

Characterization of the Escherichia coli X-ray endonuclease, endonuclease III

H L Katcher, S S Wallace


The X-ray endonuclease endonuclease III of Escherichia coli has been purified to apparent homogeneity by using the criterion of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The most purified fraction shows endonucleolytic activity against apurinic and apyrimidinic (AP) sites and a dose-dependent response to DNA that has been X irradiated, UV irradiated, or treated with OsO4. The endonuclease also nicks OsO4-treated DNA that has been subsequently treated with alkali to produce fragmented thymine residues and DNA treated with potassium permanganate. The enzyme does not incise the alkali-labile sites present in DNA X irradiated in vitro in the presence of hydroxyl radical scavengers. The most purified fractions exhibit two distinct activities, an AP endonuclease that cleaves on the 3' side of the damage leaving a 3'-OH and a 5'-PO4 and a DNA N-glycosylase that recognizes at least two substrates, thymine glycol residues and urea residues. The glycosylase activity is sensitive to N-ethylmaleimide while the AP endonuclease is not.


Sep 8, 1978·Science·I Fridovich
Sep 1, 1978·Nucleic Acids Research·P R Armel, S S Wallace
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G F Strniste, S S Wallace
Nov 6, 1974·Biochemical and Biophysical Research Communications·P V Hariharan, P A Cerutti
Apr 28, 1972·Journal of Molecular Biology·P V Hariharan, P A Cerutti
Jul 16, 1970·Biochimica Et Biophysica Acta·S Iida, H Hayatsu
Jan 1, 1966·The Biochemical Journal·K Burton, W T Riley
Sep 18, 1980·Nature·B F Demple, S Linn
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H R WarnerS Linn
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·E C FriedbergR A Schultz
Feb 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M V LafleurH Loman


Oct 1, 1984·Cell Biology and Toxicology·L Margulies, S S Wallace
Apr 19, 1985·Biochimica Et Biophysica Acta·R B Guyer, R A Deering
Jan 1, 1989·Free Radical Biology & Medicine·R Y Denq, I Fridovich
Jan 1, 1991·Free Radical Biology & Medicine·B C WhiteL I Grossman
Sep 1, 1990·Mutation Research·P W Doetsch, R P Cunningham
Mar 1, 1990·Mutation Research·Y W KowB Van Houten
Nov 26, 2003·Mutation Research·Svein Bjelland, Erling Seeberg
Jul 30, 2002·Journal of Molecular Biology·Xiang Liu, Rabindra Roy
Aug 1, 1998·Mutation Research·B J GlassnerL D Samson
Jun 28, 2002·Free Radical Biology & Medicine·S S Wallace
Feb 1, 1997·Current Opinion in Structural Biology·D G Vassylyev, K Morikawa
Aug 2, 2003·DNA Repair·Dmitry O ZharkovA P Grollman
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M C HollsteinB N Ames
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R P Cunningham, B Weiss
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y W Kow, S S Wallace
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E Chan, B Weiss
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T R O'Connor, J Laval
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J J Tsai-WuA L Lu
Jan 24, 1986·Nucleic Acids Research·R C Hayes, J E LeClerc
Sep 25, 1987·Nucleic Acids Research·G R Stuart, R W Chambers
Jun 25, 1991·Nucleic Acids Research·T Ganguly, N J Duker
Feb 13, 2001·Nucleic Acids Research·H YangJ H Miller
Nov 16, 2011·Nucleic Acids Research·Line NilsenIngrun Alseth
Jan 1, 1993·Journal of Internal Medicine·R W PeroM E Smulson
Jun 15, 1992·European Journal of Biochemistry·I HuqD E Helland
Jul 29, 1994·Annals of the New York Academy of Sciences·R P CunninghamJ A Tainer
Jun 1, 1987·Photochemistry and Photobiology·R B Weiss, N J Duker
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·C D MolJ A Tainer
Aug 1, 1990·Environmental Health Perspectives·B EpeD Wild
May 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Shane R NelsonS S Wallace
May 1, 1992·Journal of Neurochemistry·B C WhiteL I Grossman
Feb 1, 1985·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·J Cadet, M Berger
Oct 24, 2007·DNA Repair·Nora Goosen, Geri F Moolenaar
Jun 30, 2007·Journal of Inorganic Biochemistry·Amie K BoalJacqueline K Barton
Oct 15, 2013·Environmental and Molecular Mutagenesis·S S Wallace
Dec 1, 1992·Chemico-biological Interactions·B EpeC R Saha-Möller
Feb 15, 2005·Biochemical and Biophysical Research Communications·Sang Hwa KimJoon Kim
Jan 1, 1991·Chemico-biological Interactions·B Epe
Jan 1, 1983·Environmental Mutagenesis·S S Wallace
Jan 1, 1988·Environmental and Molecular Mutagenesis·S S Wallace
Jan 1, 1987·Environmental and Molecular Mutagenesis·A Eisenstark
Nov 30, 1987·Biochemical and Biophysical Research Communications·K LeeP W Doetsch
Oct 1, 2016·Journal of Biomolecular Structure & Dynamics·Nikita A KuznetsovOlga I Lavrik
Jul 16, 1985·Biochemistry·L Breimer, T Lindahl
May 16, 1989·Biochemistry·R P CunninghamM H Emptage
Nov 20, 1990·Biochemistry·R J BoorsteinG W Teebor
Oct 3, 1999·The Journal of Biological Chemistry·P M WrightA L Lu
Feb 26, 2000·Carcinogenesis·L J Marnett
Nov 17, 2001·The Journal of Biological Chemistry·Sarah BurgessR S Lloyd
Aug 10, 2000·The Journal of Biological Chemistry·F PetronzelliA Bellacosa
Sep 27, 1996·The Journal of Biological Chemistry·A L LuJ Cillo
Oct 3, 2003·The Journal of Biological Chemistry·Xiang LiuRabindra Roy
Aug 15, 1994·Molecular & General Genetics : MGG·J J Tsai-Wu, A L Lu
Apr 1, 1987·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R Téoule
Mar 25, 2008·Journal of Radiation Research·Ryohei YamamotoKihei Kubo
Sep 24, 2019·Frontiers in Plant Science·T Roldan-ArjonaDolores Córdoba-Cañero
Mar 31, 2021·International Journal of Radiation Biology·S S Wallace

Related Concepts

Endonuclease III
DNA Polymerase I
DNA, Viral
Dose-Response Relationship, Radiation
Alkalescens-Dispar Group
Plasma Protein Binding Capacity
Escherichia coli Proteins
Endonuclease V

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.