Characterization of the Escherichia coli X-ray endonuclease, endonuclease III
The X-ray endonuclease endonuclease III of Escherichia coli has been purified to apparent homogeneity by using the criterion of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The most purified fraction shows endonucleolytic activity against apurinic and apyrimidinic (AP) sites and a dose-dependent response to DNA that has been X irradiated, UV irradiated, or treated with OsO4. The endonuclease also nicks OsO4-treated DNA that has been subsequently treated with alkali to produce fragmented thymine residues and DNA treated with potassium permanganate. The enzyme does not incise the alkali-labile sites present in DNA X irradiated in vitro in the presence of hydroxyl radical scavengers. The most purified fractions exhibit two distinct activities, an AP endonuclease that cleaves on the 3' side of the damage leaving a 3'-OH and a 5'-PO4 and a DNA N-glycosylase that recognizes at least two substrates, thymine glycol residues and urea residues. The glycosylase activity is sensitive to N-ethylmaleimide while the AP endonuclease is not.
Properties of the main endonuclease specific for apurinic sites of Escherichia coli (endonuclease VI). Mechanism of apurinic site excision from DNA
Age-dependent excision repair of damaged thymine from gamma-irradiated DNA by isolated nuclei from human fibroblasts
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis
The incision and strand rejoining step in the excision repair of 5,6-dihydroxy-dihydrothymine by crude E. coli extracts
Determination of base sequence in nucleic acids with the electron microscope. V. The thymine-specific reactions of osmium tetroxide with deoxyribonucleic acid and its components
Alkali-labile sites in biologically active DNA: comparison of radiation induced potential breaks and apurinic sites
A DNA glycosylase from Escherichia coli that releases free urea from a polydeoxyribonucleotide containing fragments of base residues
Characterization of a mutation conferring radiation sensitivity, ior, located close to the gene coding for deoxycytidine deaminase in Escherichia coli
How heterologously expressed Escherichia coli genes contribute to understanding DNA repair processes in Saccharomyces cerevisiae
Regulation of a novel cell differentiation-associated gene, JWA during oxidative damage in K562 and MCF-7 cells
Identification and initial characterisation of a pyrimidine dimer UV endonuclease (UV endonuclease beta) from Deinococcus radiodurans; a DNA-repair enzyme that requires manganese ions
Enhanced transforming activity of pSV2 plasmids in human cells depends upon the type of damage introduced into the plasmid
Use of repair endonucleases to characterize DNA damage induced by reactive oxygen species in cellular and cell-free systems
An endonuclease activity of Escherichia coli that specifically removes 8-hydroxyguanine residues from DNA
Oxidative DNA base damage induced by singlet oxygen and photosensitization: recognition by repair endonucleases and mutagenicity
Reversible inactivation of E. coli endonuclease III via modification of its [4Fe-4S] cluster by nitric oxide
Physical association of the 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase of Escherichia coli and an activity nicking DNA at apurinic/apyrimidinic sites
Escherichia coli MutY protein has both N-glycosylase and apurinic/apyrimidinic endonuclease activities on A.C and A.G mispairs
Novel nuclear and mitochondrial glycosylases revealed by disruption of the mouse Nth1 gene encoding an endonuclease III homolog for repair of thymine glycols
Enzymatic excision from gamma-irradiated polydeoxyribonucleotides of adenine residues whose imidazole rings have been ruptured
Dihydrothymidine and thymidine glycol triphosphates as substrates for DNA polymerases: differential recognition of thymine C5-C6 bond saturation and sequence specificity of incorporation
The mechanisms of action of E. coli endonuclease III and T4 UV endonuclease (endonuclease V) at AP sites
Phylogenetic evidence of a role for 5-hydroxymethyluracil-DNA glycosylase in the maintenance of 5-methylcytosine in DNA
Cloning and characterization of the mvrC gene of Escherichia coli K-12 which confers resistance against methyl viologen toxicity
Analysis of DNA damage and repair in murine leukemia L1210 cells using a quantitative polymerase chain reaction assay
Solution conformation of an oligonucleotide containing a urea deoxyribose residue in front of a thymine
Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum
Molecular cloning and functional analysis of a Schizosaccharomyces pombe homologue of Escherichia coli endonuclease III
Efficient recognition of substrates and substrate analogs by the adenine glycosylase MutY requires the C-terminal domain
Single Qdot-labeled glycosylase molecules use a wedge amino acid to probe for lesions while scanning along DNA
Positively charged residues within the iron-sulfur cluster loop of E. coli MutY participate in damage recognition and removal
Two glycosylase families diffusively scan DNA using a wedge residue to probe for and identify oxidatively damaged bases
Effect of rec mutations on viability and processing of DNA damaged by methylmethane sulfonate in xth nth nfo cells of Escherichia coli
Electrochemistry of nonconjugated proteins and glycoproteins. Toward sensors for biomedicine and glycomics
DNA repair glycosylases with a [4Fe-4S] cluster: a redox cofactor for DNA-mediated charge transport?
A new Schizosaccharomyces pombe base excision repair mutant, nth1, reveals overlapping pathways for repair of DNA base damage
DNA damage by oxygen radicals and excited state species: a comparative study using enzymatic probes in vitro
Crystallization and crystallographic characterization of the iron-sulfur-containing DNA-repair enzyme endonuclease III from Escherichia coli
Quantitation of radiation-, chemical-, or enzyme-induced single strand breaks in nonradioactive DNA by alkaline gel electrophoresis: application to pyrimidine dimers
Host cell reactivation of plasmids containing oxidative DNA lesions is defective in Cockayne syndrome but normal in UV-sensitive syndrome fibroblasts
The mechanism of human tyrosyl-DNA phosphodiesterase 1 in the cleavage of AP site and its synthetic analogs
Thymine glycol-DNA glycosylase/AP endonuclease of CEM-C1 lymphoblasts: a human analog of Escherichia coli endonuclease III
The excision of AP sites by the 3'-5' exonuclease activity of the Klenow fragment of Escherichia coli DNA polymerase I
The human homolog of Escherichia coli endonuclease V is a nucleolar protein with affinity for branched DNA structures
Pre-steady state kinetics of DNA binding and abasic site hydrolysis by tyrosyl-DNA phosphodiesterase 1
Urea Mimics Nucleobases by Preserving the Helical Integrity of B-DNA Duplexes via Hydrogen Bonding and Stacking Interactions
UV-induced pyrimidine hydrates in DNA are repaired by bacterial and mammalian DNA glycosylase activities
A new mechanism for repairing oxidative damage to DNA: (A)BC excinuclease removes AP sites and thymine glycols from DNA
Ultraviolet-induced thymine hydrates in DNA are excised by bacterial and human DNA glycosylase activities
Damage repertoire of the Escherichia coli UvrABC nuclease complex includes abasic sites, base-damage analogues, and lesions containing adjacent 5' or 3' nicks
Substrate specificity of the Escherichia coli endonuclease III: excision of thymine- and cytosine-derived lesions in DNA produced by radiation-generated free radicals
DNA base and strand damage in X-irradiated monkey CV-1 cells: influence of pretreatment using small doses of radiation
Methanobacterium thermoformicicum thymine DNA mismatch glycosylase: conversion of an N-glycosylase to an AP lyase
Enzymatic repair of 5-formyluracil. I. Excision of 5-formyluracil site-specifically incorporated into oligonucleotide substrates by alka protein (Escherichia coli 3-methyladenine DNA glycosylase II).
The C-terminal domain of MutY glycosylase determines the 7,8-dihydro-8-oxo-guanine specificity and is crucial for mutation avoidance
Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase
Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue.
Purification and cloning of Micrococcus luteus ultraviolet endonuclease, an N-glycosylase/abasic lyase that proceeds via an imino enzyme-DNA intermediate.
Effect of expression of the Escherichia coli nth gene in Saccharomyces cerevisiae on the toxicity of ionizing radiation and hydrogen peroxide
Purification and characterization of a novel DNA repair enzyme from the extremely radioresistant bacterium Rubrobacter radiotolerans
Molecular Radiobiology and the Origins of the Base Excision Repair Pathway: An Historical Perspective.
Toward a detailed understanding of base excision repair enzymes: transition state and mechanistic analyses of N-glycoside hydrolysis and N-glycoside transfer
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