Abstract
A cytochrome P-450 system in Rhodococcus strains, encoded by thcB, thcC, and thcD, participates in the degradation of thiocarbamates and several other pesticides. The regulation of the system was investigated by fusing a truncated lacZ in frame to thcB, the structural gene for the cytochrome P-450 monooxygenase. Analysis of the thcB-lacZ fusion showed that the expression of thcB was 10-fold higher in the presence of the herbicide EPTC (s-ethyl dipropylthiocarbamate). Similar enhancement of the thcB-lacZ expression was found with other thiocarbamate pesticides. Atrazine, simazine, or carbofuran, although metabolized by the system, had no effect on the thcB-lacZ expression. The presence of glucose slightly increased the expression of thcB-lacZ, indicating no catabolic repression of the thcB-lacZ expression. The expression of thcB-lacZ was decreased more than twofold in Luria-Bertani medium. This was due in part to cysteine, which repressed thcB-lacZ expression. It was confirmed that the thcR gene, which is transcribed divergently from thcB, codes for a positive regulatory protein which is essential for the thcB-lacZ expression. Studies of the thcR-lacZ protein fusion showed that the thcR gene is expressed constitutively.
References
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B Vogelstein, D Gillespie
Jan 1, 1992·Annual Review of Microbiology·W R Finnerty
Mar 1, 1992·Gene·N V Patel, C A Omer
May 1, 1992·Journal of General Microbiology·Y HashimotoT Beppu
Sep 1, 1991·Molecular Microbiology·D P O'Keefe, P A Harder
Jan 1, 1991·Advances in Applied Microbiology·F S Sariaslani
Feb 16, 1991·Biochimica Et Biophysica Acta·Y HashimotoT Beppu
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·F P Guengerich
May 15, 1989·European Journal of Biochemistry·O IkehataT Beppu
May 1, 1987·Applied and Environmental Microbiology·A C TamS U Khan
Nov 1, 1983·Gene·S K ShapiraM J Casadaban
Oct 1, 1995·Letters in Applied Microbiology·Z ShaoR M Behki
Oct 1, 1995·Journal of Bacteriology·Z Q ShaoR M Behki
May 1, 1995·Applied and Environmental Microbiology·I NagyR De Mot
May 1, 1995·Applied and Environmental Microbiology·Z Q Shao, R Behki
Feb 1, 1995·Journal of Bacteriology·I NagyR de Mot
Jan 1, 1994·Critical Reviews in Biotechnology·A M Warhurst, C A Fewson
Jun 1, 1993·Applied and Environmental Microbiology·R BehkiP Germon
Mar 1, 1993·Journal of Bacteriology·U KarlsonL D Eltis
Feb 25, 1993·Nucleic Acids Research·M T GallegosJ L Ramos
Apr 1, 1993·European Journal of Biochemistry·L D EltisK N Timmis
Sep 1, 1990·Applied and Environmental Microbiology·J DesomerM V Montagu
Sep 1, 1993·Applied and Environmental Microbiology·S A DenomeK D Young
Citations
Apr 14, 2012·The Journal of Biological Chemistry·Soi H BuiAndrew W Munro
Feb 2, 2018·Critical Reviews in Biotechnology·Bo JiangDayi Zhang
Jul 14, 2001·FEMS Microbiology Ecology·S RousseauxG Soulas
Jan 22, 2017·Environmental Science and Pollution Research International·R L YaleJ W B Moir
Jan 9, 1998·Journal of Bacteriology·M J SadowskyL P Wackett
Oct 29, 1998·Journal of Bacteriology·V S Kalogeraki, S C Winans
May 4, 2017·Ecotoxicology·Judit HáhnBalázs Kriszt
Aug 2, 2003·FEMS Microbiology Letters·René De MotAnnabel H A Parret
Apr 7, 2021·Biotechnology Advances·Youxiang Liang, Huimin Yu
Apr 24, 2021·Chemosphere·Sandhya MishraShaohua Chen
Jan 1, 1997·Plasmid·H ZhengJ F Prescott
May 1, 1997·Applied and Environmental Microbiology·A De SchrijverR De Mot