Characterization of the expression of the thcB gene, coding for a pesticide-degrading cytochrome P-450 in Rhodococcus strains.

Applied and Environmental Microbiology
Z Q Shao, R Behki

Abstract

A cytochrome P-450 system in Rhodococcus strains, encoded by thcB, thcC, and thcD, participates in the degradation of thiocarbamates and several other pesticides. The regulation of the system was investigated by fusing a truncated lacZ in frame to thcB, the structural gene for the cytochrome P-450 monooxygenase. Analysis of the thcB-lacZ fusion showed that the expression of thcB was 10-fold higher in the presence of the herbicide EPTC (s-ethyl dipropylthiocarbamate). Similar enhancement of the thcB-lacZ expression was found with other thiocarbamate pesticides. Atrazine, simazine, or carbofuran, although metabolized by the system, had no effect on the thcB-lacZ expression. The presence of glucose slightly increased the expression of thcB-lacZ, indicating no catabolic repression of the thcB-lacZ expression. The expression of thcB-lacZ was decreased more than twofold in Luria-Bertani medium. This was due in part to cysteine, which repressed thcB-lacZ expression. It was confirmed that the thcR gene, which is transcribed divergently from thcB, codes for a positive regulatory protein which is essential for the thcB-lacZ expression. Studies of the thcR-lacZ protein fusion showed that the thcR gene is expressed constitutively.

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Citations

Apr 14, 2012·The Journal of Biological Chemistry·Soi H BuiAndrew W Munro
Feb 2, 2018·Critical Reviews in Biotechnology·Bo JiangDayi Zhang
Jan 22, 2017·Environmental Science and Pollution Research International·R L YaleJ W B Moir
Apr 7, 2021·Biotechnology Advances·Youxiang Liang, Huimin Yu
May 1, 1997·Applied and Environmental Microbiology·A De SchrijverR De Mot

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