Characterization of the fecal and mucosa-associated microbiota in dogs with colorectal epithelial tumors

PloS One
Kristin Marie Valand HerstadEllen Skancke

Abstract

Colorectal epithelial tumors occur spontaneously in dogs, and the pathogenesis seems to parallel that of humans. The development of human colorectal tumorigenesis has been linked to alterations in the composition of the intestinal microbiota. This study characterized the fecal- and mucosa-associated microbiota in dogs with colorectal epithelial tumors (n = 10). The fecal microbiota was characterized by 16S rDNA analysis and compared with that of control dogs (n = 13). We also determined the mucosa-associated microbiota composition in colonic tumor tissue (n = 8) and in adjacent non-tumor tissue (n = 5) by 16S rDNA- and rRNA profiling. The fecal microbial community structure in dogs with tumors was different from that of control samples and was distinguished by oligotypes affiliated with Enterobacteriaceae, Bacteroides, Helicobacter, Porphyromonas, Peptostreptococcus and Streptococcus, and lower abundance of Ruminococcaceae, Slackia, Clostridium XI and Faecalibacterium. The overall community structure and populations of mucosal bacteria were not different based on either the 16S rDNA or the 16S rRNA profile in tumor tissue vs. adjacent non-tumor tissue. However, the proportion of live, potentially active bacteria appeared to be ...Continue Reading

References

Oct 17, 1991·The New England Journal of Medicine·J ParsonnetR K Sibley
Sep 1, 1988·The New England Journal of Medicine·B VogelsteinJ L Bos
Mar 1, 1997·Journal of the American Animal Hospital Association·K D ValeriusS J Withrow
Jul 25, 1998·Gastroenterology·A SwidsinskiH Lochs
May 20, 1999·Veterinary Pathology·M F McEntee, K A Brenneman
Jun 21, 2002·The Journal of Small Animal Practice·J M DobsonJ L N Wood
Jul 23, 2004·The Journal of Small Animal Practice·M CravenM L Chandler
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Jul 18, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·N Ulger ToprakG Soyletir
Jul 25, 2006·Infection and Immunity·Kenneth W SimpsonYnte H Schukken
May 15, 2007·World Journal of Surgical Oncology·Mary JonesJoseph Mathew
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Mar 7, 2008·European Journal of Epidemiology·Sven HasslerLena Damber
Dec 17, 2009·FEMS Microbiology Ecology·Anne-Mette R GrønvoldRoderick I Mackie
Jan 21, 2010·Genome Research·Jie TangShaying Zhao
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Apr 28, 2010·Gastroenterology·Kory W JaspersonRandall W Burt
Aug 20, 2010·Inflammatory Bowel Diseases·S MondotM Leclerc
Jan 21, 2011·Journal of Experimental & Clinical Cancer Research : CR·Ahmed S AbdulamirFatimah Abu Bakar
Jun 8, 2011·PloS One·Julian R MarchesiHarold Tjalsma
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Jun 28, 2011·Genome Biology·Nicola SegataCurtis Huttenhower
Oct 20, 2011·Genome Research·Aleksandar D KosticMatthew Meyerson
Apr 4, 2012·Journal of AOAC International·George T Macfarlane, Sandra Macfarlane
Jun 26, 2012·Nature Reviews. Microbiology·Harold TjalsmaBas E Dutilh
Aug 17, 2012·Nutrition Reviews·Willem M de Vos, Elisabeth A J de Vos
Oct 19, 2012·Cancer Metastasis Reviews·Robert L Johnson, James C Fleet
Apr 5, 2013·The American Journal of Clinical Nutrition·Hui-Min ChenJing-Yuan Fang

❮ Previous
Next ❯

Citations

Aug 31, 2020·Microbial Biotechnology·Giulia AlessandriMarco Ventura
Sep 6, 2019·Helicobacter·Armelle Ménard, Annemieke Smet
Jun 19, 2019·PloS One·Nihar Ranjan DashMohammad Tahseen Al Bataineh
Oct 21, 2020·Microorganisms·Nadia BykovaSergey Musienko
Nov 3, 2020·Frontiers in Veterinary Science·Ana NemecNataša Tozon
Jun 22, 2021·Critical Reviews in Microbiology·Sofia Ochoa, Luis Collado

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP110343
PRJNA391562

Methods Mentioned

BETA
biopsy
PCR
electrophoresis

Software Mentioned

UCHIME
ANOSIM
QIIME
Agilent 2100 Expert Software
vegan
SDS
PRIMER
PRINSEQ lite
R phyloseq
PERMANOVA

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.