Characterization of the First α-(1→3) Branching Sucrases of the GH70 Family.

The Journal of Biological Chemistry
Marlène VuilleminM Remaud-Simeon

Abstract

Leuconostoc citreumNRRL B-742 has been known for years to produce a highly α-(1→3)-branched dextran for which the synthesis had never been elucidated. In this work a gene coding for a putative α-transglucosylase of the GH70 family was identified in the reported genome of this bacteria and functionally characterized. From sucrose alone, the corresponding recombinant protein, named BRS-B, mainly catalyzed sucrose hydrolysis and leucrose synthesis. However, in the presence of sucrose and a dextran acceptor, the enzyme efficiently transferred the glucosyl residue from sucrose to linear α-(1→6) dextrans through the specific formation of α-(1→3) linkages. To date, BRS-B is the first reported α-(1→3) branching sucrase. Using a suitable sucrose/dextran ratio, a comb-like dextran with 50% of α-(1→3) branching was synthesized, suggesting that BRS-B is likely involved in the comb-like dextran produced byL. citreumNRRL B-742. In addition, data mining based on the search for specific sequence motifs allowed the identification of two genes putatively coding for branching sucrases in the genome ofLeuconostoc fallaxKCTC3537 andLactobacillus kunkeeiEFB6. Biochemical characterization of the corresponding recombinant enzymes confirmed their branc...Continue Reading

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Citations

Jan 22, 2019·Critical Reviews in Microbiology·Pasquale FilanninoMarco Gobbetti
Oct 7, 2019·Applied Microbiology and Biotechnology·Yuqing TianWanmeng Mu
Oct 13, 2018·Scientific Reports·Yannick MalbertMagali Remaud-Simeon
Apr 11, 2020·Biotechnology Advances·Claire BourlieuGabriel Paës
Apr 4, 2021·International Journal of Molecular Sciences·Karan WangpaiboonRath Pichyangkura
Dec 16, 2021·Critical Reviews in Food Science and Nutrition·Xiaodan LiWeifeng Liu

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