Characterization of the function of the ver-1A and ver-1B genes, involved in aflatoxin biosynthesis in Aspergillus parasiticus.

Applied and Environmental Microbiology
S H LiangJ E Linz

Abstract

The ver-1A gene was cloned and its nucleotide sequence was determined as part of a previous study on aflatoxin B1 (AFB1) biosynthesis in the filamentous fungus Aspergillus parasiticus SU-1. A second copy of this gene, ver-1B, was tentatively identified in this fungal strain. In this study, ver-1B was cloned by screening an A. parasiticus cosmid library with a ver-1A probe. The nucleotide sequence of ver-1B was determined. The predicted amino acid sequence of ver-1B had 95% identity with ver-1A. A translational stop codon, found in the ver-1B gene coding region, indicated that it encodes a truncated polypeptide. To confirm the function of the ver-1 genes in AFB1 synthesis, a plasmid (pDV-VA) was designed to disrupt ver-1A and/or ver-1B by transformation of the AFB1 producer A. parasiticus NR-1. One disruptant, VAD-102, which accumulated the pathway intermediate versicolorin A was obtained. Southern hybridization analysis of VAD-102 revealed that ver-1A but not ver-1B was disrupted. A functional ver-1A gene was transformed back into strain VAD-102. Transformants which received ver-1A produced AFB1, confirming that ver-1A is the only functional ver-1 gene in A. parasiticus SU-1 and that its gene product is involved in the conversi...Continue Reading

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Citations

Mar 12, 2004·Annual Review of Phytopathology·G A Payne, M P Brown
Apr 1, 2004·The Journal of Biological Chemistry·Ludmila V RozeJohn E Linz
Jun 4, 2005·Applied and Environmental Microbiology·Emi SakunoHiromitsu Nakajima
Aug 6, 2004·Applied and Environmental Microbiology·Ana M CalvoNancy P Keller
Mar 10, 2004·Applied and Environmental Microbiology·Jiujiang YuJoan W Bennett
Mar 29, 2007·Natural Product Reports·Dirk Hoffmeister, Nancy P Keller
Jul 1, 2005·Molecular Plant-microbe Interactions : MPMI·Tami McDonaldThomas M Hammond
Apr 9, 1998·FEMS Microbiology Letters·C P Woloshuk, R Prieto
Dec 12, 2012·Applied Microbiology and Biotechnology·Tamás PappCsaba Vágvölgyi
Jun 1, 2002·Applied and Environmental Microbiology·Rosie E BradshawJanet M Seconi
Dec 8, 2005·Applied and Environmental Microbiology·Kenneth C EhrlichDeepak Bhatnagar
Jul 31, 2002·Applied and Environmental Microbiology·Tadashi TakahashiYasuji Koyama
Jan 5, 2018·Molecular & Cellular Proteomics : MCP·Silin RenShihua Wang
May 18, 1999·Fungal Genetics and Biology : FG & B·M P BrownG A Payne
Oct 31, 2000·Applied and Environmental Microbiology·P K ChangT E Cleveland
Mar 1, 1997·Applied and Environmental Microbiology·S H LiangJ E Linz
Jun 16, 2017·Environmental Science & Technology·Chandrani MitraAnindya Chanda
Oct 9, 2008·Biotechnology Advances·David Lubertozzi, Jay D Keasling
Mar 10, 2005·Journal of the American Chemical Society·Kevin M Henry, Craig A Townsend
May 27, 2011·Applied Microbiology and Biotechnology·Ludmila V RozeJohn E Linz

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