Characterization of the gastric motility response to human motilin and erythromycin in human motilin receptor-expressing transgenic mice

PloS One
Shinichi KatoBunzo Matsuura

Abstract

Motilin is a gastrointestinal peptide hormone that stimulates gastrointestinal motility. Motilin is produced primarily in the duodenum and jejunum. Motilin receptors (MTLRs) are G protein-coupled receptors that may represent a clinically useful pharmacological target as they can be activated by erythromycin. The functions of motilin are highly species-dependent and remain poorly understood. As a functional motilin system is absent in rodents such as rats and mice, these species are not commonly used for basic studies. In this study, we examine the usefulness of human MTLR-overexpressing transgenic (hMTLR-Tg) mice by identifying the mechanisms of the gastric motor response to human motilin and erythromycin. The distribution of hMTLR was examined immunohistochemically in male wild-type (WT) and hMTLR-Tg mice. The contractile response of gastric strips was measured isometrically in an organ bath, while gastric emptying was determined using phenol red. hMTLR expression was abundant in the gastric smooth muscle layer. Interestingly, higher levels of hMTLR expression were observed in the myenteric plexus of hMTLR-Tg mice but not WT mice. hMTLR was not co-localized with vesicular acetylcholine transporter, a marker of cholinergic neur...Continue Reading

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Citations

Jul 14, 2020·Current Opinion in Infectious Diseases·Nicholas A TurnerChristopher R Polage
Apr 3, 2019·International Journal of Molecular Sciences·Tomoe KawamuraYoichi Hiasa
Jan 5, 2021·Current Opinion in Endocrinology, Diabetes, and Obesity·Ichiro Sakata, Shota Takemi

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Methods Mentioned

BETA
transgenic

Software Mentioned

Elements AR
GraphPad
NIS
GraphPad Prism

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