PMID: 8606150Apr 1, 1996Paper

Characterization of the gene coding for GDP-mannose dehydrogenase (algD) from Azotobacter vinelandii

Journal of Bacteriology
M CamposG Soberón-Chávez

Abstract

Azotobacter vinelandii presents a differentiation process leading to the formation of desiccation-resistant cysts. Alginate, the exopolysaccharide produced by this bacterium, has been postulated to have a role in cyst formation. Here, we report the cloning and characterization of the A. vinelandii gene coding for the enzyme GDP-mannose dehydrogenase (algD), which is the key enzyme for alginate synthesis in Pseudomonas aeruginosa. This gene has a high degree of similarity with the algD gene from P. aeruginosa, and similar proteins seem to be involved in algD regulation in both bacteria. We show the existence of two mRNA start sites; one of these sites corresponds to a promoter transcribed by RNA polymerase containing a sigma E subunit. An A. vinelandii algD mutant which is completely impaired in alginate production and which is unable to form desiccation-resistant cells was constructed. The effects of NH4, NO3, and NaCl concentrations on algD transcription for three A. vinelandii strains producing different alginate levels were evaluated. We found a strict correlation between alginate production and algD transcription for the three strains studied; however, the effects on algD transcription under the conditions studied were diff...Continue Reading

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Citations

Jan 1, 1997·Critical Reviews in Biotechnology·F Clementi
Aug 17, 2006·Biotechnology Letters·Uwe Remminghorst, Bernd H A Rehm
Nov 20, 2008·Archives of Microbiology·Gabriela García-EsquivelFernando José Esparza-García
Jan 17, 2014·Environmental Microbiology·Iain D HayBernd H A Rehm
Jun 16, 2015·Frontiers in Microbiology·Helga Ertesvåg
Jun 2, 2015·Frontiers in Microbiology·Gregory B WhitfieldP Lynne Howell
Dec 13, 2016·FEMS Microbiology Letters·Adan TrejoGuadalupe Espín
Jan 31, 2012·Journal of Molecular Microbiology and Biotechnology·Joaquín ManzoMiguel Castañeda
Aug 11, 2012·Applied Microbiology and Biotechnology·Cinthia NúñezGuadalupe Espín
Nov 1, 2016·Applied Microbiology and Biotechnology·Carlos Leonel Ahumada-ManuelCinthia Núñez
Mar 7, 2007·Molecular Genetics and Genomics : MGG·Thirunavukkarasu NagarajanAnil Kumar Tripathi
Nov 14, 2000·Journal of Bacteriology·C NúñezG Soberón-Chávez
Dec 29, 1998·Journal of Bacteriology·C NúñezG Espín
Jun 6, 1998·Journal of Bacteriology·S MorenoG Espín
Jul 1, 1997·Journal of Bacteriology·A Peñaloza-VázquezC L Bender

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