Characterization of the gene delivery properties of baculoviral-based virosomal vectors

Journal of Virological Methods
Ghiabe-Henri GuibingaTheodore Friedmann

Abstract

The current study reports the production of baculoviral-virosomal vectors consisting of lipoplexes and of the viral glycoprotein (GP64) of baculovirus Autographa californica multiple nucleopolyhdrovirus (AcMNPV). This study demonstrates that such complexes have an increased transfection capability in a number of cells, including undifferentiated H9 human embryonic stem H9hES cells compared to lipoplexes alone. The GP64-mediated enhancement of gene transfer of lipoplexes is inhibited by the addition of anti-GP64 neutralizing antibody and by a modified GP64 protein, but is however less potent than vesicular stomatitis virus glycoprotein (VSV-G)-mediated enhancement of gene transfer of lipoplexes. This difference may be explained in part by the dissimilarity in the fusogenic properties of their respective viral glycoprotein.

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Citations

Feb 25, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Minna U KaikkonenKari J Airenne
Aug 2, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Bin ShiLaura Sepp-Lorenzino
Mar 19, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Bin Shi, Marc Abrams

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