PMID: 2122457Nov 1, 1990Paper

Characterization of the gene encoding the protective paracrystalline-surface-layer protein of Rickettsia prowazekii: presence of a truncated identical homolog in Rickettsia typhi

Proceedings of the National Academy of Sciences of the United States of America
M CarlG A Dasch

Abstract

The DNA sequence of the gene encoding the protective surface protein antigen (SPA) of Rickettsia prowazekii has been determined. The open reading frame of 4836 nucleotides with promoter and ribosome-binding site is present on a 10.1-kilobase EcoRI fragment. The encoded carboxyl terminus of the 169-kDa protein contains a potential transmembrane region and hydrophilic regions with many lysine and arginine residues potentially accessible to proteolytic cleavage. Because the rickettsia-derived SPA has an estimated molecular mass of only 120 kDa and does not contain several predicted large carboxyl-region CNBr fragments, the SPA product appears to be processed by the rickettsiae. Eight other CNBr fragments were identical in sequence to those predicted from the encoded gene. A complementary 8.7-kilobase EcoRI fragment of Rickettsia typhi DNA was cloned. This fragment lacked a 1433-base-pair region that included the promoter, ribosome-binding site, and the initial 1162 base pairs of the open reading frame encoding the R. prowazekii SPA but had a 3674-base-pair region identical with the remainder of the R. prowazekii SPA gene sequence.

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Apr 1, 1990·The Journal of Infectious Diseases·M CarlG A Dasch
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Citations

Jan 13, 2006·Journal of Molecular Evolution·Francis M Jiggins
Jan 21, 2000·FEMS Microbiology Reviews·L A Fernández, J Berenguer
Mar 1, 1997·Veterinary Microbiology·S McOristD G Smith
Feb 17, 2001·Infection and Immunity·I R Henderson, J P Nataro
Jan 29, 2000·Journal of Bacteriology·M Sára, U B Sleytr
Jul 1, 1997·Emerging Infectious Diseases·D RaoultR Birtles
Jan 1, 1990·Annals of the New York Academy of Sciences·M CarlG A Dasch
Jun 23, 2009·Annals of the New York Academy of Sciences·Victor V Emelyanov
Jul 16, 2003·Annals of the New York Academy of Sciences·Ju JiangAllen L Richards
Apr 11, 2007·FEMS Microbiology Letters·Hong GeAllen L Richards
Feb 17, 2006·Annals of the New York Academy of Sciences·Tsuneo Uchiyama
Jun 1, 1997·FEMS Microbiology Reviews·H BahlS Resch
Apr 29, 1998·Journal of Bacteriology·L I RachekD O Wood

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