PMID: 2509430Nov 1, 1989Paper

Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis

Journal of Bacteriology
M PeregoJ A Hoch

Abstract

The kinA (spoIIJ) locus contains a single gene which codes for a protein of 69,170 daltons showing strong homology to the transmitter kinases of two component regulatory systems. The purified kinase autophosphorylates in the presence of ATP and mediates the transfer of phosphate to the Spo0A and Spo0F sporulation regulatory proteins. Spo0F protein was a much better phosphoreceptor for this kinase than Spo0A protein in vitro. Mutants with deletion mutations in the kinA gene were delayed in their sporulation. They produced about a third as many spores as the wild type in 24 h, but after 72 h on solid medium, the level of spores approximated that found for the wild-type strain. Such mutations had no effect on the regulation of the abrB gene or on the timing of subtilisin expression and therefore did not impair the repression function of the Spo0A protein. Placement of the kinA locus on a multicopy vector suppressed the sporulation-defective phenotype of spo0B, spo0E, and spo0F mutations but not of spo0A mutations. The results suggest that the spo0B-, spo0E-, and spo0F-dependent pathway of activation (phosphorylation) of the Spo0A regulator may be by-passed through the kinA gene product if it is present at sufficiently high intrace...Continue Reading

References

Jan 1, 1976·Advances in Genetics·J A Hoch
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A J Ninfa, B Magasanik
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K A TrachJ A Hoch
Dec 1, 1985·Journal of Bacteriology·V GrisoliaC B Bruni
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B T NixonF M Ausubel
Dec 1, 1988·Journal of Bacteriology·T SekiH Saito
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F A FerrariJ A Hoch
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E C Kofoid, J S Parkinson
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J F HessM I Simon
Jun 1, 1983·Journal of Bacteriology·F A FerrariJ A Hoch
May 25, 1984·Nucleic Acids Research·V Brendel, E N Trifonov
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen

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Citations

Aug 1, 1991·Molecular Microbiology·C MathiopoulosA L Sonenshein
Jan 1, 1990·Critical Reviews in Biotechnology·M M Nakano, P Zuber
Jan 1, 1991·Molecular Microbiology·D Dubnau
Aug 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Stock
Mar 1, 1993·Molecular Microbiology·M A MarahielP Zuber
Mar 1, 1997·Critical Reviews in Toxicology·J C Rowlands, J A Gustafsson
Nov 26, 2010·Journal of Bacteriology·Anna L McLoonRichard Losick
Jun 10, 2014·FEMS Microbiology Letters·Adrianne N Edwards, Shonna M McBride
Jun 2, 2016·Journal of Bacteriology·Kathryn L NawrockiShonna M McBride
Sep 10, 2017·Annual Review of Microbiology·James A Hoch
Mar 13, 2018·PLoS Pathogens·Brintha Parasumanna GirinathanRevathi Govind
May 22, 2019·Annual Review of Microbiology·Vanessa I Francis, Steven L Porter
Aug 1, 1991·Molecular Microbiology·F J SlackA L Sonenshein
Feb 24, 2001·Annals of the New York Academy of Sciences·J L KielJ G Bruno
Jun 1, 1997·Genome Research·J Michaud, C M Fan
Dec 25, 2009·FEMS Microbiology Reviews·Daniel López, Roberto Kolter
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Catherine BurkeTorsten Thomas
Mar 15, 2015·Frontiers in Microbiology·Josephine E E U HellbergGeorge P C Salmond
Jan 30, 2015·Microbiology and Molecular Biology Reviews : MMBR·Mohab A Al-HinaiEleftherios T Papoutsakis
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J F BarrettJ A Hoch
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·Y Z GuC A Bradfield
Sep 21, 2016·Infection and Immunity·Kevin O ChildressShonna M McBride
Jun 7, 2017·Journal of Bacteriology·Ramses Gallegos-MonterrosaÁkos T Kovács

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