PMID: 8969471Jun 1, 1996Paper

Characterization of the gene for leucomyosuppressin and its expression in the brain of the cockroach Diploptera punctata

Insect Biochemistry and Molecular Biology
B C DonlyW G Bendena

Abstract

Using HPLC separation, radioimmunoassay, and subsequent bioassay, we have detected the presence of an active peptide, which co-elutes with the insect myoinhibitory peptide leuco-myosuppressin, in the brain of the cockroach Diploptera punctata. We have isolated a cDNA encoding the precursor for this peptide from cDNA libraries representing D. punctata brain RNA. The cDNA sequence contains an open reading frame that upon translation would result in a prepropolypeptide of 96 amino acids. Proteolytic cleavage of the predicted precursor could result in several peptides, including a 10 amino acid C-terminal peptide that would, upon modification of the NH2 and COOH-terminal amino acids, be identical to the insect FLRFamide, leucomyosuppressin. No other RFamide products are predicted to be processed from the precursor. Southern blot analysis indicates that the gene is present in the D. punctata genome in a single copy. Northern blot analysis shows that the gene is predominantly expressed as a 3.8 kb mRNA in cockroach brain. Study of the expression of the leucomyosuppressin gene in D. punctata brain, using in situ hybridization, indicates that expression occurs primarily in the pars intercerebralis of the protocerebrum, a region showing...Continue Reading

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Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B C DonlyW G Bendena

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Citations

Jan 25, 2000·Archives of Insect Biochemistry and Physiology·J A Veenstra
Feb 17, 2001·Peptides·R PredelM Eckert
Apr 9, 1998·Peptides·A B Lange, I Orchard
Jan 24, 2002·Insect Biochemistry and Molecular Biology·Tabita MalutanCam Donly
Sep 14, 2006·Annual Review of Entomology·Barbara Stay, Stephen S Tobe
Jan 23, 2003·Microscopy Research and Technique·A Joffre MercierMaria Boldt
Apr 16, 2002·The Journal of Comparative Neurology·Dan LuJane L Witten
Sep 9, 2008·General and Comparative Endocrinology·N Audsley, R J Weaver

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