PMID: 8606151Apr 1, 1996Paper

Characterization of the genes coding for the putative sigma factor AlgU and its regulators MucA, MucB, MucC, and MucD in Azotobacter vinelandii and evaluation of their roles in alginate biosynthesis

Journal of Bacteriology
J Martínez-SalazarV Deretic

Abstract

The study of the biosynthesis of alginate, the exopolysaccharide produced by Azotobacter vinelandii and Pseudomonas aeruginosa, has biotechnological and medical significance. We report here the identification of the A. vinelandii genes coding for the putative sigma factor AlgU and its negative regulators MucA and MucB through the suppression of the highly mucoid phenotype of an A. vinelandii strain by a plasmid encoding MucA and MucB. The sequences of the A. vinelandii algU, mucA, and mucB genes are highly homologous to those of the corresponding P. aeruginosa genes, AlgU shows 93% identity, and MucA and MucB are 64.4 and 63.9% identical, respectively. Forming part of the same operon as algU, mucA, and mucB, two additional genes (mucC and mucD) were identified and sequenced; the product of the former gene is homologous to ORF4 of Photobacterium sp. strain SS9, and that of the latter gene belongs to the HtrA serine protease family. Interestingly, the nonmucoid A. vinelandii UW136 had a 0.9-kb insertion within the algU gene. A strong correlation between AlgU activity and alginate production by A. vinelandii was also found, as reflected in the level of algD transcription.

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Citations

Aug 11, 2012·Applied Microbiology and Biotechnology·Cinthia NúñezGuadalupe Espín
Jan 18, 2017·Molecular Microbiology·Luis Felipe Muriel-MillánGuadalupe Espín
Jul 13, 2017·Microbiology·Elva Quiroz-RochaCinthia Nuñez
Mar 14, 2018·Microbiology·Jesse D Noar, Jose M Bruno-Bárcena
Jul 29, 1998·Molecular Microbiology·D Missiakas, S Raina
Mar 27, 2002·DNA Sequence : the Journal of DNA Sequencing and Mapping·L A PrestonN L Schiller
Jan 1, 1997·Critical Reviews in Biotechnology·F Clementi
Jan 31, 2012·Journal of Molecular Microbiology and Biotechnology·Joaquín ManzoMiguel Castañeda
Jun 11, 2015·PloS One·Robert L RobsonToby H Richardson
Jan 5, 2002·DNA Sequence : the Journal of DNA Sequencing and Mapping·L M Keith, C L Bender
Nov 14, 2000·Journal of Bacteriology·C NúñezG Soberón-Chávez
Aug 20, 2004·Journal of Bacteriology·Stephen E LizewskiMichael J Schurr
Dec 29, 1998·Journal of Bacteriology·C NúñezG Espín
Jun 6, 1998·Journal of Bacteriology·S MorenoG Espín

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