Characterization of the genes responsible for rubber degradation in Actinoplanes sp. strain OR16

Applied Microbiology and Biotechnology
Namiko GibuDaisuke Kasai

Abstract

A Gram-positive rubber-degrading bacterium, Actinoplanes sp. strain OR16 (strain NBRC 114529), is able to grow on agar plates containing natural and synthetic rubber as the sole sources of carbon and energy. When this strain was grown on natural rubber latex overlay agar plates, translucent halos around the cells were observed. To identify the natural rubber degradation genes and other features of its metabolism, its complete genome sequence was determined. The genome of OR16 consists of 9,293,892 bp and comprises one circular chromosome (GenBank accession number AP019371.1) with a G + C content of 70.3%. The genome contains 8238 protein-coding and 18 rRNA genes. A homology search of the genome sequence revealed that three genes (lcp1, lcp2, and lcp3) are homologous to an extracellular latex-clearing protein (Lcp) of Streptomyces sp. K30. RT-PCR analysis revealed that lcp1 and lcp2 seem to constitute an operon. Purified lcp gene products have oxygen consumption activity toward natural rubber latex, suggesting that all these genes encode rubber-degrading enzymes in OR16. Quantitative reverse transcription-PCR analysis indicated that the transcription of these genes is induced during the growth of OR16 on natural rubber. The gene...Continue Reading

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Citations

Jul 3, 2021·Polymers·Ann Anni BasikKumar Sudesh

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Datasets Mentioned

BETA
AP019371.1

Methods Mentioned

BETA
PCR
reverse transcription-PCR
electrophoresis
column chromatography
electrophoretic mobility shift

Software Mentioned

TatP
tBLASTn
JSpecies Web Service

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