PMID: 2506593Jul 1, 1989Paper

Characterization of the genetic element coding for lactose metabolism in Lactococcus lactis subsp. lactis KP3

Plasmid
J L SteeleL L McKay

Abstract

The Lactococcus lactis subsp. lactis KP3 Lac genetic element was investigated. KP3 is a lactose-positive (Lac+) transconjugant which contains no detectable plasmid DNA. The KP3 Lac genetic element was self-transmissible (Tra+) and encoded a reduced bacteriophage sensitivity (Rbs+) phenotype. Matings of KP3 with a recombination-deficient (Rec-) recipient resulted in Lac+ transconjugants which were phenotypically indistinguishable from KP3 and contained a 96-MDa plasmid (pJS96). Phenotypic and physical analyses of pJS96 indicated that it was a deletion derivative of a putative pKB32::pJS88 Lac+ Tra+ cointegrate. pKB32 is the Lac plasmid and pJS88 is the Tra+ Rbs+ plasmid in L. lactis subsp. lactis 11007, the donor used in obtaining KP3. The results presented suggest that pJS96 is an episome, since it appeared to replicate both as a plasmid and as an integrated part of the chromosome. Conjugal transfer of chromosomal DNA mediated by pJS96 was not observed. Conjugal transfer of pJS96 resulted in Lac+ transconjugants containing plasmids ranging in size from 21 to 90 MDa. Only in Rec+ recipients were transconjugants isolated which appeared to contain pJS96 integrated into the host chromosome. Restriction analysis of several plasmids ...Continue Reading

References

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Citations

Jul 1, 1991·The Journal of Applied Bacteriology·C J Pillidge, L E Pearce
Jan 1, 1993·Journal of Dairy Science·D A Romero, T R Klaenhammer
Oct 1, 1994·FEMS Microbiology Reviews·W M de Vos, E E Vaughan
Sep 1, 1990·FEMS Microbiology Reviews·M J Gasson
Jun 1, 1990·Applied and Environmental Microbiology·A J HaandrikmanG Venema

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