PMID: 2494359Apr 1, 1989Paper

Characterization of the genome of Pseudomonas aeruginosa bacteriophage phi PLS27 with particular reference to the ends of the DNA

Journal of Virology
B J AllanA M Kropinski

Abstract

The DNA of Pseudomonas aeruginosa rough-specific bacteriophage phi PLS27 was studied. The genome size as determined by summing the sizes of restriction fragments was 42.7 kilobase pairs. Of particular interest was the fact that the DNA was insensitive to certain common restriction endonucleases including EcoRI, BamHI, and HindIII. The ends of the phage DNA were cloned and sequenced, revealing direct repeats of 318 nucleotides. The left end of the genome when cloned into the promoter selection vector pKK232-8 exhibited promoter activity in Escherichia coli. Two promoters bearing greater than 70% sequence homology to the plasmid pNM74 TOL operon and PAK pilin promoters were identified.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 1, 1986·Journal of Bacteriology·B B FinlayW Paranchych
Sep 1, 1987·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·B Allan, A M Kropinski
Jan 1, 1987·CRC Critical Reviews in Biochemistry·A A Travers
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G L GrayH L Heyneker
Jun 1, 1981·Analytical Biochemistry·D S Holmes, M Quigley
Jan 30, 1983·Virology·H Fujisawa, K Sugimoto
Apr 15, 1983·European Journal of Biochemistry·M CoeneC Cocito
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·F W Studier, J J Dunn
Jun 5, 1981·Journal of Molecular Biology·J J Dunn, F W Studier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.