Characterization of the growth hormone-binding protein of human serum using a panel of monoclonal antibodies.

The Journal of Endocrinology
R BarnardM J Waters

Abstract

A panel of monoclonal antibodies (MAbs) reactive with distinct epitopes on the rabbit liver GH receptor and rabbit serum GH-binding protein (GHBP) were tested for cross-reactivity with the GHBP from human serum. Four of seven MAbs reacted with the human serum GHBP. Immunoprecipitation of the human binding protein enabled hormonal specificity identical to that previously reported for human GH receptors to be demonstrated. Scatchard analyses of 125I-labelled human GH binding to the serum GHBP were carried out with correction made for endogenous human GH which was measured by radioimmunoassay of each serum sample. This approach yielded the first reliable estimates of the affinity and capacity of the human GHBP. The binding capacity (mean +/- S.E.M.) of female sera (804 +/- 126 pmol/l; n = 6) was greater than that of male sera (505 +/- 36 pmol/l; n = 9; P less than 0.02). The affinity of the GHBP was 0.91 +/- 0.10 litres/nmol (n = 15). The presence of multiple epitopes common to the human serum GHBP and the rabbit liver GH receptor is consistent with identity between the extracellular domains of the human GHBP and the human GH receptor, as is the case for the rabbit GHBP and GH receptor.

Citations

May 1, 1994·Molecular and Cellular Endocrinology·T AmitZ Hochberg
Jun 1, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Jens FuglsangPer Ovesen
Aug 22, 2001·Molecular and Cellular Endocrinology·K TakagiJ Sawada
Jan 1, 1990·Acta Paediatrica Scandinavica. Supplement·M J WatersW I Wood
Sep 1, 1993·Acta Paediatrica. Supplement·G A WertherM J Waters
Feb 25, 2006·European Journal of Endocrinology·Jens FuglsangPer Ovesen
Jan 1, 1994·Journal of Endocrinological Investigation·G BaumannK Amburn
Apr 28, 2005·Clinical Endocrinology·Jens FuglsangPer Ovesen
Jan 1, 1997·Metabolism: Clinical and Experimental·J VittoneR G Spencer
Dec 20, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Sanne Fisker
Aug 15, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·L K ChopinA C Herington
Apr 12, 2002·Endocrine Reviews·Fernando M ReisFelice Petraglia
Aug 14, 1998·Journal of Pediatric Gastroenterology and Nutrition·R M GreerR W Shepherd
May 1, 2001·Journal of Pediatric Endocrinology & Metabolism : JPEM·G Baumann
Jan 3, 2001·The Journal of Clinical Endocrinology and Metabolism·K C LeungK K Ho
Apr 2, 2002·Journal of Pediatric Endocrinology & Metabolism : JPEM·Norma DávilaGerhard Baumann
Sep 1, 2000·Journal of Pediatric Endocrinology & Metabolism : JPEM·E CodnerD E Codner
Jan 1, 1993·Journal of Receptor Research·R G Duggleby, M J Waters

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