Characterization of the haemolytic activity of Streptococcus equi

Microbial Pathogenesis
J FlanaganN Chanter

Abstract

The haemolytic activity of Streptococcus equi, the cause of equine strangles, was characterized. Production of haemolysin in Todd Hewitt broth was dependent on an equine serum supplement and the logarithmic phase of growth after which activity declined sharply. RNA core also induced haemolysin production from cells harvested at the end of the logarithmic phase of growth. Haemolysis was not affected by cholesterol, was only slightly increased in reducing conditions and was completely inactivated by trypan blue, identifying the haemolytic activity as streptolysin S-like (SLS-like). Purification by hydroxyapatite and Sephacryl column chromatography yielded proteins of molecular weights of approximately 6000 and 17 000-22 000 Da with a 64-fold increase in specific activity. Low molecular weight proteins from the RNA core were still present in the purified toxin. Two non-haemolytic mutants were derived by conjugation with an Enterococcus faecalis-carrying transposon Tn916. Southern blots of HindIII digests of DNA revealed that one of the mutants contained three transposon insertions and the other just one. A lambda phage library of S. equi contained plaques whose haemolytic activity was enhanced by reducing conditions and inhibited ...Continue Reading

References

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Citations

Aug 9, 2011·Nature Reviews. Microbiology·Evelyn M MolloyR Paul Ross
May 7, 2003·Applied and Environmental Microbiology·Philip A Wescombe, John R Tagg
Oct 22, 2005·Infection and Immunity·Jonas LannergårdBengt Guss
Dec 14, 2005·Journal of the American Veterinary Medical Association·Beatrice T SponsellerJohn F Timoney
Oct 11, 2014·The Veterinary Clinics of North America. Equine Practice·Andrew S Waller
Oct 18, 2002·Veterinary Microbiology·John A Walker, John F Timoney
Jul 16, 2011·Journal of Medical Microbiology·Andrew S WallerJohn F Timoney
Nov 10, 2014·Medical Microbiology and Immunology·Daniela AsamBarbara Spellerberg
Mar 4, 2017·Chemical Reviews·Brandon J BurkhartDouglas A Mitchell
Jan 24, 2009·Equine Veterinary Journal·J F Timoney, P Kumar
Feb 9, 2016·Molecular BioSystems·Karen F StewardAndrew S Waller
Oct 4, 2015·Cellular Microbiology·Timothy C BarnettMark J Walker
Aug 5, 2000·Biochemical and Biophysical Research Communications·I AlbesaM G Paraje
Mar 16, 2019·Microbiology Spectrum·Horst Malke
May 22, 2019·Microbiology Spectrum·Claire E TurnerAndroulla Efstratiou
Jul 13, 2021·Frontiers in Immunology·Lubica DraberovaPetr Draber
Jul 27, 2021·Case Reports in Neurological Medicine·Jeroen KerstensPhilippe G Jorens

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