PMID: 8969924Nov 1, 1996Paper

Characterization of the hamster CYP11B2 gene regulatory regions

Endocrine Research
N CoulombeJ G LeHoux

Abstract

We have isolated a hamster CYP11B2 gene encoding the cytochrome P450 aldosterone synthase. In comparison with the CYP11B2 gene of other species, cis-elements named Ad1, Ad2, Ad3, and Ad4, were identified in the 5'-untranslated region of the hamster gene. Mouse adrenal tumor cells were transiently transfected with chimaeric reporter constructs, fused to the bacterial chloramphenicol acyltransferase (CAT) reporter gene, to study the regulation of expression of the hamster CYP11B2 gene. The highest basal expression was obtained with the -130 bp construct. Decreasing the length of the regulatory region of the CYP11B2 gene beyond that of -130 bp, to exclude Ad2 and Ad1 elements, resulted in successive decreases in CAT activity. Increasing the length of the regulatory region beyond that of -130 bp also resulted in a reduction of CAT activity, indicating the presence of inhibitory cis-elements in this area of the gene. Forskolin stimulated the CAT activity of all constructs, the highest of which occurred with the -130 bp construct, indicating that the gene is controlled by the PKA signalling pathway. TPA, however, had no stimulatory effects on any of these constructs. Staurosporine, an inhibitor of the PKC pathway, stimulated cells tr...Continue Reading

References

Jan 1, 1987·Journal of Steroid Biochemistry·J G LehouxD Bellabarba
Feb 1, 1983·The Journal of Clinical Endocrinology and Metabolism·G T GriffingJ C Melby
Jan 1, 1980·Journal of Steroid Biochemistry·J G Lehoux, A Lefebvre
Jun 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·J G LeHouxA Lefebvre
Dec 29, 1995·Annals of the New York Academy of Sciences·M CloutierJ G Lehoux
Jan 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·S VéronneauJ G LeHoux

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Citations

Feb 24, 2001·Endocrine Research·J G LeHouxA Lefebvre

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