PMID: 3770142Oct 1, 1986Paper

Characterization of the heat shock response in M-14 human melanoma cells continuously exposed to supranormal temperatures

Experimental and Molecular Pathology
A DelpinoU Ferrini

Abstract

The heat shock response elicited in a human melanoma cell line (M-14) by continuous exposures to supranormal temperatures has been characterized. The electrophoretic patterns of polypeptides labeled in vivo at different time-intervals during a continuous heating at 42 degrees C show that the hyperthermic stress induces the synthesis of three HSPs, with molecular weights, respectively, of 86 kDa, 70-72 kDa and 26 kDa. The relative rate of synthesis of the 70-72 kDa HSP--the preeminent HSP--increases during the first hours of treatment, reaching the maximum value after about 9 hr. Later on, the rate of synthesis of this protein progressively decreases, finally attaining a steady state level only slightly exceeding the constitutive one. On the contrary, the smaller molecular weight HSP is synthesized at an apparently constant rate in the course of 21 hr of heating treatment. A continuous exposure at 40 degrees C induces the synthesis of the same three HSPs observed in cells heated at 42 degrees C, but the rate of synthesis of all these HSPs is not so greatly enhanced over the control values as in the 42 degrees C-heated cells. Moreover, the repression of the 70-72 kDa HSP synthesis is faster, taking place within 4-6 hr of treatmen...Continue Reading

References

Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S L McKenzieM Meselson
Jan 1, 1969·Journal of Molecular Biology·W McCormick, S Penman
Sep 1, 1983·Experimental Cell Research·E M Berger, M P Woodward
Feb 25, 1980·Journal of Molecular Biology·S Lindquist

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