Characterization of the hemoglobin of the backswimmer Anisops deanei (Hemiptera)

Insect Biochemistry and Molecular Biology
Agnes WawrowskiThorsten Burmester

Abstract

While O(2)-binding hemoglobin-like proteins are present in many insects, prominent amounts of hemoglobin have only been found in a few species. Backswimmers of the genera Anisops and Buenoa (Notonectidae) have high concentrations of hemoglobin in the large tracheal cells of the abdomen. Oxygen from the hemoglobin is delivered to a gas bubble and controls the buoyant density, which enables the bugs to maintain their position without swimming and to remain stationary in the mid-water zone where they hunt for prey. We have obtained the cDNA sequences of three Anisops deanei hemoglobin chains by RT-PCR and RACE techniques. The deduced amino acid sequences show an unusual insertion of a single amino acid in the conserved helix E, but this does not affect protein stability or ligand binding kinetics. Recombinant A. deanei hemoglobin has an oxygen affinity of P(50) = 2.4 kPa (18 torr) and reveals the presence of a dimeric fraction or two different conformations. The absorption spectra demonstrate that the Anisops hemoglobin is a typical pentacoordinate globin. Phylogenetic analyses show that the backswimmer hemoglobins evolved within Heteroptera and most likely originated from an intracellular hemoglobin with divergent function.

References

Sep 16, 1992·Biochemical and Biophysical Research Communications·P A OsmulskiP G Debrunner
Apr 14, 1986·Biochemical and Biophysical Research Communications·R O RyanJ H Law
Jul 1, 1969·British Journal of Haematology·D R Miller
Nov 26, 1998·The Journal of Biological Chemistry·S DewildeL Moens
Dec 22, 1999·Molecular Biology and Evolution·T Burmester, T Hankeln
Aug 29, 2001·Bioinformatics·J P Huelsenbeck, F Ronquist
Mar 28, 2002·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·T Burmester
Jun 6, 2002·The Journal of Biological Chemistry·Thomas HankelnThorsten Burmester
May 29, 2003·Journal of Insect Physiology·M Locke
Dec 24, 2003·The Journal of Experimental Biology·Kendra J Greenlee, Jon F Harrison
Dec 24, 2003·The Journal of Experimental Biology·Kendra J Greenlee, Jon F Harrison
Jan 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Silke Hagner-HollerThorsten Burmester
May 27, 2005·The Journal of Biological Chemistry·Daniele de SanctisMartino Bolognesi
Nov 2, 2005·Protein Science : a Publication of the Protein Society·Alessandra PesceMartino Bolognesi
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Jan 20, 2006·The FEBS Journal·Thorsten BurmesterThomas Hankeln
May 12, 2006·Nature·Philip G D Matthews, Roger S Seymour
May 16, 2006·Journal of Insect Physiology·Thomas HankelnThorsten Burmester
Jan 1, 1946·The Biochemical Journal·D Keilin, Y L Wang
Feb 17, 2007·Journal of Insect Physiology·Thorsten Burmester, Thomas Hankeln
Sep 6, 2008·Insect Biochemistry and Molecular Biology·Christian PickThorsten Burmester
Sep 18, 2008·The FEBS Journal·Eva GleixnerThomas Hankeln
Dec 2, 2008·The Journal of Experimental Biology·Philip G D Matthews, Roger S Seymour
Feb 25, 2009·The FEBS Journal·Christian PickThorsten Burmester
Sep 29, 2011·Journal of Insect Physiology·Philip G D Matthews, Roger S Seymour

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Citations

Dec 22, 2015·Wilderness & Environmental Medicine·James H Diaz
Mar 24, 2016·Insect Biochemistry and Molecular Biology·Eva GleixnerThomas Hankeln
Jul 2, 2015·Integrative and Comparative Biology·Thorsten Burmester
Oct 6, 2016·Environmental Toxicology and Chemistry·Yeisson GutiérrezEugênio E Oliveira
Jun 6, 2020·PloS One·Hollister W HerholdDavid A Grimaldi
Sep 15, 2020·Frontiers in Genetics·Andreas ProthmannThomas Hankeln
Nov 6, 2015·The Journal of Experimental Biology·Karl K JonesRoger S Seymour

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