Characterization of the Highly Autolytic Lactococcus lactis subsp. cremoris Strains CO and 2250.

Applied and Environmental Microbiology
H R RiepeL L McKay

Abstract

Two highly autolytic Lactococcus lactis subsp. cremoris strains (CO and 2250) were selected and analyzed for their autolytic properties. Both strains showed maximum lysis when grown in M17 broth containing a limiting concentration of glucose (0.4 to 0.5%) as the carbohydrate source. Lysis did not vary greatly with pH or temperature but was reduced when strains were grown on lactose or galactose. Growth in M17 containing excess glucose (1%) prevented autolysis, although rapid lysis of L. lactis subsp. cremoris CO did occur in the presence of 1% glucose if sodium fluoride (an inhibitor of glycolysis) was added to the medium. Maximum cell lysis in a buffer system was observed early in the stationary phase, and for CO, two pH optima were observed for log-phase and stationary-phase cells (6.5 and 8.5, respectively). Autolysins were extracted from the cell wall fraction of each strain by using either 4% sodium dodecyl sulfate (SDS), 6 M guanidine hydrochloride, or 4 M lithium chloride, and their activities were analyzed by renaturing SDS-polyacrylamide gel electrophoresis on gels containing Micrococcus luteus or L. lactis subsp. cremoris CO cells as the substrate. More than one lytic band was observed on each substrate, with the majo...Continue Reading

References

Sep 1, 1977·Antimicrobial Agents and Chemotherapy·R J LesherR E Marquis
Jun 1, 1976·The Journal of Dairy Research·J J SullivanL Mou
Apr 1, 1988·Biochimie·G A Somkuti, D H Steinberg
Apr 1, 1973·Journal of Bacteriology·J Coyette, G D Shockman
Aug 1, 1969·Journal of Bacteriology·L L McKayP R Elliker
Mar 1, 1980·Journal of Bacteriology·L K JolliffeU N Streips
Sep 1, 1983·Applied and Environmental Microbiology·D G Anderson, L L McKay
Mar 1, 1988·Applied and Environmental Microbiology·Ian B PowellBarrie E Davidson
Jun 1, 1975·Applied Microbiology·B E Terzaghi, W E Sandine
Sep 1, 1995·Applied and Environmental Microbiology·F Valence, S Lortal

❮ Previous
Next ❯

Citations

Feb 7, 2001·Applied and Environmental Microbiology·R CibikM P Chapot-Chartier
Feb 2, 2000·Applied and Environmental Microbiology·C Husson-KaoM P Chapot-Chartier
Feb 11, 2015·Annual Review of Food Science and Technology·Larry McKay
Sep 24, 2016·Applied Microbiology and Biotechnology·Ganesh Ram R VisweswaranGirbe Buist
Jun 3, 2021·Foods·Tahl Zimmerman, Salam A Ibrahim

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.