Characterization of the human peripheral lung adenosine receptor

American Journal of Respiratory Cell and Molecular Biology
J P Joad

Abstract

Because adenosine narrows asthmatic airways, is released during hypoxia and by mast cells, and is antagonized by theophylline, it may play a role in asthma. I characterized the first step in pulmonary responses to adenosine: its adenosine receptor. Plasma membranes, prepared from macroscopically normal human peripheral lung, were incubated with 10 nM 5'-N-ethylcarboxamido[3H]adenosine ([3H]NECA) and various concentrations of competing ligand under experimentally determined optimal conditions: 4 degrees C, pH 7.4, 5 mM MgCl2, 1.8 mg protein/ml, 30-min incubation time. Bound and free ligand were separated by rapid vacuum filtration, and the radioactive counts were analyzed using a weighted, non-linear, least-squares curve-fitting program, LIGAND. Analyzed together, the data from the lungs of 6 patients revealed a single binding site with a dissociation constant (Kd) for NECA of 200 nM +/- 14% and a receptor concentration of 543 fmol/mg protein +/- 13%. Analyzed separately, the individual Kds ranged from 133 to 430 nM and the receptor concentrations from 338 to 811 fmol/mg protein. Adenosine receptor ligands competed with NECA in an A2 rank order of potency: NECA greater than 8-phenyltheophylline greater than 3-isobutyl-1-methylxa...Continue Reading

References

Jan 7, 2000·Pediatric Research·G G KonduriS Mital
Mar 4, 2008·British Journal of Pharmacology·R A BrownC P Page
Jul 16, 2011·European Journal of Pharmacology·Daniela ImpellizzeriSalvatore Cuzzocrea
Sep 1, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S R RutgersD S Postma
Feb 1, 1990·American Journal of Respiratory Cell and Molecular Biology·T H Steinberg
Jul 1, 1993·The American Review of Respiratory Disease·A G DriverS J Mustafa
Aug 1, 1993·American Journal of Respiratory Cell and Molecular Biology·J P Joad, K S Kott
Mar 6, 2004·The Journal of Biological Chemistry·Hisayo InbeJeffrey A Encinas

Citations

Dec 11, 1975·The American Journal of Physiology·R M MentzerR M Berne
Feb 1, 1985·The Journal of Allergy and Clinical Immunology·M J Cushley, S T Holgate
Oct 1, 1985·Naunyn-Schmiedeberg's Archives of Pharmacology·D UkenaU Schwabe
Sep 1, 1985·Agents and Actions·D L Marquardt, S I Wasserman
Mar 1, 1988·Biochemical Pharmacology·J P Joad, T B Casale
Aug 1, 1987·The American Review of Respiratory Disease·R A Pauwels, M E Van der Straeten
May 1, 1985·British Journal of Clinical Pharmacology·J S Mann, S T Holgate
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Mar 1, 1982·Journal of Medicinal Chemistry·J W Daly
Feb 1, 1983·British Journal of Clinical Pharmacology·M J CushleyS T Holgate
May 1, 1984·The Journal of Allergy and Clinical Immunology·M Weinberger
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D L MarquardtS I Wasserman

Related Concepts

Methyladenine Receptors
Receptors, Purinergic P1
Filtration
Vicrom
Adenosine
Lung
Mast Cell
Adenosine-5'-(N-ethylcarboxamide)
1-Methyl-3-isobutylxanthine
Ligand Binding Domain

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