Characterization of the human peripheral lung adenosine receptor

American Journal of Respiratory Cell and Molecular Biology
J P Joad


Because adenosine narrows asthmatic airways, is released during hypoxia and by mast cells, and is antagonized by theophylline, it may play a role in asthma. I characterized the first step in pulmonary responses to adenosine: its adenosine receptor. Plasma membranes, prepared from macroscopically normal human peripheral lung, were incubated with 10 nM 5'-N-ethylcarboxamido[3H]adenosine ([3H]NECA) and various concentrations of competing ligand under experimentally determined optimal conditions: 4 degrees C, pH 7.4, 5 mM MgCl2, 1.8 mg protein/ml, 30-min incubation time. Bound and free ligand were separated by rapid vacuum filtration, and the radioactive counts were analyzed using a weighted, non-linear, least-squares curve-fitting program, LIGAND. Analyzed together, the data from the lungs of 6 patients revealed a single binding site with a dissociation constant (Kd) for NECA of 200 nM +/- 14% and a receptor concentration of 543 fmol/mg protein +/- 13%. Analyzed separately, the individual Kds ranged from 133 to 430 nM and the receptor concentrations from 338 to 811 fmol/mg protein. Adenosine receptor ligands competed with NECA in an A2 rank order of potency: NECA greater than 8-phenyltheophylline greater than 3-isobutyl-1-methylxa...Continue Reading


Jan 7, 2000·Pediatric Research·G G KonduriS Mital
Mar 4, 2008·British Journal of Pharmacology·R A BrownC P Page
Jul 16, 2011·European Journal of Pharmacology·Daniela ImpellizzeriSalvatore Cuzzocrea
Sep 1, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S R RutgersD S Postma
Feb 1, 1990·American Journal of Respiratory Cell and Molecular Biology·T H Steinberg
Jul 1, 1993·The American Review of Respiratory Disease·A G DriverS J Mustafa
Aug 1, 1993·American Journal of Respiratory Cell and Molecular Biology·J P Joad, K S Kott
Mar 6, 2004·The Journal of Biological Chemistry·Hisayo InbeJeffrey A Encinas


Dec 11, 1975·The American Journal of Physiology·R M MentzerR M Berne
Feb 1, 1985·The Journal of Allergy and Clinical Immunology·M J Cushley, S T Holgate
Oct 1, 1985·Naunyn-Schmiedeberg's Archives of Pharmacology·D UkenaU Schwabe
Sep 1, 1985·Agents and Actions·D L Marquardt, S I Wasserman
Mar 1, 1988·Biochemical Pharmacology·J P Joad, T B Casale
Aug 1, 1987·The American Review of Respiratory Disease·R A Pauwels, M E Van der Straeten
May 1, 1985·British Journal of Clinical Pharmacology·J S Mann, S T Holgate
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Mar 1, 1982·Journal of Medicinal Chemistry·J W Daly
Feb 1, 1983·British Journal of Clinical Pharmacology·M J CushleyS T Holgate
May 1, 1984·The Journal of Allergy and Clinical Immunology·M Weinberger
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D L MarquardtS I Wasserman

Related Concepts

Methyladenine Receptors
Receptors, Purinergic P1
Mast Cell
Ligand Binding Domain

Related Feeds


This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.

Related Papers

American Journal of Respiratory Cell and Molecular Biology
J P Joad, K S Kott
Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition
G MandalariSalvatore Cuzzocrea
The Journal of Asthma : Official Journal of the Association for the Care of Asthma
Jose BeldaJoaquín Sanchis
Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
R LeverC P Page
© 2020 Meta ULC. All rights reserved