Characterization of the human somatostatin receptor type 4 promoter

Molecular and Cellular Endocrinology
Stephan PetersennH M Schulte

Abstract

Somatostatin (SRIF) exerts inhibitory effects on virtually all endocrine and exocrine secretions. Five distinct SRIF receptor subtypes (sst 1-5) have been identified. In contrast to the other subtypes, very little is known about specific functions of sst4. We investigated structure and regulation of the human sst4 gene. A genomic clone containing the 5' region of the sst4 gene was isolated. 1.5 kb of the promoter was sequenced and putative transcription factor binding sites were identified. The transcription start site was located 88 nucleotides upstream of the translation start site. A -984 sst4 promoter directed significant levels of luciferase expression in GH4 rat pituitary cells, Skut-1B endometrium cells, and BEAS-2B human bronchial epithelial cells, whereas only low activity was detected in JEG3 chorion carcinoma cells or COS-7 monkey kidney cells. A minimal -209 promoter allowed cell specific expression, its activity in COS-7 cells is not enhanced by co-transfection of the pituitary-specific transcription factor Pit-1. An enhancer element was localized between nt -459 and -984. We did not find any regulation of the sst4 promoter region analyzed by SRIF, forskolin, TPA, IGF-1, EGF, T3, glucocorticoids or 17beta-estradiol...Continue Reading

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Citations

Apr 28, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Massimo Dal MontePaola Bagnoli
Oct 11, 2011·Cell Biology International·Dewi Kenneth RowlandsHsiao Chang Chan
Jul 18, 2008·Social Neuroscience·Chi-Fu Jeffrey YangHarrison G Pope
May 10, 2007·Developmental Neuropsychology·Karen Chipman, Elizabeth Hampson
Dec 23, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John M CoatesZoltan Sarnyai

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