PMID: 11342220May 9, 2001Paper

Characterization of the human UDP-galactose:ceramide galactosyltransferase gene promoter

Biochimica Et Biophysica Acta
T TencomnaoD Kapitonov

Abstract

UDP-galactose:ceramide galactosyltransferase (CGT, EC 2.4.1.45) is a key enzyme in the biosynthesis of galactocerebroside, the most abundant glycosphingolipid in the myelin sheath. An 8 kb fragment upstream from the transcription initiation site of CGT gene was isolated from a human genomic DNA library. Primer extension analysis revealed a single transcription initiation site 329 bp upstream from the ATG start codon. Neither a consensus TATA nor a CCAAT box was identified in the proximity to the transcription start site; however, this region contains a high GC content and multiple putative regulatory elements. To investigate the transcriptional regulation of CGT, a series of 5' deletion constructs of the 5'-flanking region were generated and cloned upstream from the luciferase reporter gene. By comparing promoter activity in the human oligodendroglioma (HOG) and human neuroblastoma (LAN-5) cell lines, we found that the CGT promoter functions in a cell type-specific manner. Three positive cis-acting regulatory regions were identified, including a proximal region at -292/-256 which contains the potential binding sites for known transcription factors (TFs) such as Ets and SP1 (GC box), a distal region at -747/-688 comprising a num...Continue Reading

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Citations

Mar 14, 2012·Neurochemical Research·Robert K YuToshio Ariga
Nov 11, 2011·International Journal of Molecular Sciences·Anchalee PrasansuklabTewin Tencomnao
Apr 19, 2005·Colloids and Surfaces. B, Biointerfaces·Hiromichi NakaharaOsamu Shibata
Apr 17, 2004·Journal of Lipid Research·Robert K YuGuichao Zeng
Jul 30, 2005·Colloids and Surfaces. B, Biointerfaces·Tomoki MarutaOsamu Shibata

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