Characterization of the immunomodulatory properties of alveolar bone-derived mesenchymal stem cells.

Stem Cell Research & Therapy
Chen CaoD Kaigler

Abstract

Recently, mesenchymal stem cells (MSCs) have been shown to have immunomodulatory properties which hold promise for their clinical use to treat inflammatory conditions. Relative to bone marrow-derived MSCs (BMSCs), which are typically isolated from the iliac crest, we have recently demonstrated that MSCs can be predictably isolated from the alveolar bone (aBMSCs) by less invasive means. As such, the aim of this study was to characterize the immunomodulatory properties of aBMSCs relative to BMSCs. aBMSCs isolated from the human alveolar bone and BMSCs isolated from the human bone marrow of the iliac crest were cultured in the same conditions. Cytokine arrays and enzyme-linked immunosorbent assays (ELISA) of a conditioned medium were used to evaluate differences in the secretion of cytokines. In different functional assays, aBMSCs and BMSCs were cocultured with different types of immune cells including THP-1 monocytes, macrophages, and peripheral blood mononuclear cells (PBMCs) to evaluate their effects on important immune cell functions including proliferation, differentiation, and activation. The protein arrays identified interleukin (IL)-6 and monocyte chemoattractant protein (MCP)-1 to be the major cytokines secreted by aBMSCs...Continue Reading

References

Feb 2, 2002·Experimental Hematology·Amelia BartholomewRonald Hoffman
Dec 8, 2004·Stem Cells·Pieternella S In 't AnkerHumphrey H H Kanhai
Mar 5, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takehiro MatsubaraYukio Kato
Mar 22, 2005·Experimental Cell Research·Ida RasmussonKatarina Le Blanc
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Simon A Jones
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·R M DwyerM J Kerin
Oct 13, 2007·Stem Cells·Mariane SeccoMayana Zatz
Sep 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Federica CasiraghiMarina Noris
May 16, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Satish L DeshmaneBassel E Sawaya
Sep 24, 2009·Experimental Hematology·Jaehyup Kim, Peiman Hematti
Mar 5, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takashi KikuiriSongtao Shi
Oct 29, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Antony J CutlerCristina V Navarrete
Nov 16, 2010·Journal of Dental Research·T YamazaS Shi
Oct 16, 2012·Current Stem Cell Research & Therapy·Mandana Mohyeddin BonabMohammad Reza Gheini
Apr 20, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Monika K GrudzinskaPiotr Religa
Oct 31, 2013·Journal of Dental Research·S MasonD Kaigler
Nov 13, 2013·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Joanne KurtzbergSonali Chaudhury
Jan 8, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Toshio TakanoToshio Kukita
Mar 19, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elena Sierra-FilardiAngel L Corbí
Apr 22, 2015·Nature Immunology·Christopher A Hunter, Simon A Jones
Aug 13, 2015·BMC Medicine·Naomi D'souzaMassimo Dominici
Aug 16, 2016·International Journal of Cell Biology·Ranjeet Singh Mahla

❮ Previous
Next ❯

Citations

Apr 27, 2021·Journal of Clinical Periodontology·Danae A ApatzidouAntonis Konstantinidis

❮ Previous
Next ❯

Methods Mentioned

BETA
bone marrow aspirations
ELISA
Assay
flow cytometry
PMA
flow
FCS
protein

Software Mentioned

Prism
GraphPad
FCS Express
Prochymal

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.