PMID: 9423878Jan 10, 1998Paper

Characterization of the immunostimulatory properties of Leishmania infantum HSP70 by fusion to the Escherichia coli maltose-binding protein in normal and nu/nu BALB/c mice

Infection and Immunity
A I RicoJ M Requena

Abstract

Leishmania infantum HSP70 has been described as an immunodominant antigen in both humans and dogs suffering from visceral leishmaniasis. In this study, we used L. infantum HSP70 fused to Escherichia coli maltose-binding protein (MBP), as the reporter protein, to analyze the influence of HSP70 on the immunogenicity of MBP in BALB/c mice. Plasmids were constructed to produce the three recombinant proteins used in this study, namely, MBP, L. infantum HSP70, and MBP-HSP70, which consists of MBP fused to the L. infantum HSP70 amino terminus. Immunization of BALB/c mice with the MBP-HSP70 fusion protein elicited humoral and cellular responses against MBP that were higher by an order of magnitude than those elicited by immunization with MBP alone or with a mixture of MBP and HSP70. Covalent linkage of MBP to HSP70 was essential for eliciting a strong anti-MBP immune response. Cytokine secretion and immunoglobulin G isotype analyses indicated that immunization with the MBP-HSP70 fusion protein preferentially induces a Th1 immune response. Immunization of athymic nu/nu mice with the MBP-HSP70 fusion protein unexpectedly gave rise to an anti-MBP humoral response showing features of a T-cell-dependent response. Thus, we present evidence t...Continue Reading

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Citations

Nov 25, 2003·Cell Stress & Chaperones·Udayasankar KumaraguruBarry D Bruce
Oct 11, 2008·Expert Review of Vaccines·Azam Bolhassani, Sima Rafati
Mar 26, 2003·Cell Stress & Chaperones·Ana I RicoJose M Requena
Dec 13, 2016·Transactions of the Royal Society of Tropical Medicine and Hygiene·Anuradha Ratna, Sunil K Arora
Sep 5, 2020·Advanced Healthcare Materials·Eduardo Ruiz-HitzkyPilar Aranda
Feb 28, 2019·Frontiers in Veterinary Science·Valeria A SanderMarina Clemente
Mar 7, 2021·Veterinary Sciences·Ehab Kotb Elmahallawy, Abdulsalam A M Alkhaldi
Oct 16, 2008·Journal of Immunological Methods·Martin LarsenPeter Kristensen

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